Detection of influenza A virus RNA in birds by optimized Real-Time PCR system

被引：0
作者：
Ilinykh, Ph A. [1 ]
Shestopalova, E. M. [1 ]
Khripko, Yu, I [1 ]
Durimanov, A. G. [1 ]
Sharshov, K. A. [1 ]
Shestopalov, A. M. [1 ]
机构：
[1] State Res Ctr Virol & Biotechnol Vector, Koltsov 630559, Novosibirsk Reg, Russia
来源：
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2010年 / 3卷 / 05期
关键词：
Influenza A virus RNA; Real-Time PCR; MDCK cell culture; Virus surveillance; Wild birds; WILD MIGRATORY BIRDS; AVIAN INFLUENZA; QUANTITATIVE PCR; H5N1; IDENTIFICATION; PREVALENCE; PATTERNS; SUBTYPES; ALASKA; ASSAY;
D O I：
10.1016/S1995-7645(10)60082-X
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Objective: To evaluate the use of Real Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds. Methods: Sensitivity, specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed. Results: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MOCK cell culture. Conclusions: Real-time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.
引用
收藏
页码：337 / 340
页数：4
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