Manipulating the molecular weight of alginate produced by Azotobacter vinelandii in continuous cultures

被引：18

作者：

Diaz-Barrera, Alvaro ^{[1
]}

Silva, Paulina ^{[1
]}

Berrios, Julio ^{[1
]}

Acevedo, Fernando ^{[1
]}

机构：

[1] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Valparaiso, Chile

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 23期

关键词：

Alginate; Molecular weight; Dilution rate; Chemostat; Sucrose uptake rate; DISSOLVED-OXYGEN; PHOSPHATE; AGITATION; YIELD; LYASE; MASS;

D O I：

10.1016/j.biortech.2010.07.038

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Alginate production by Azotobacter vinelandii in chemostat cultures was evaluated at different dilution rates (D) and inlet sucrose concentrations of 5 and 20 g l(-1). At the low inlet sucrose concentration, the molecular weight of alginate increased from 800 to 1800 kDa when D increased from 0.05 to 0.10 h(-1), whereas the opposite trend was observed with the high inlet sucrose concentration. This behaviour can be explained by changes in specific sucrose uptake rate. Thus, a decrease in alginate molecular weight was dependent on the specific sucrose uptake rate when this rate was higher than 0.42 g g(-1) h(-1). The manipulation of the D can be used to select the molecular weight of alginate in continuous culture. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.

引用

页码：9405 / 9408

页数：4

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