C-type natriuretic peptide plasma levels and whole blood mRNA expression show different trends in adolescents with different degree of endothelial dysfunction

被引:11
作者
Del Ry, Silvia [1 ,3 ]
Cabiati, Manuela [1 ]
Bianchi, Vanessa [2 ]
Randazzo, Emioli [2 ]
Peroni, Diego [2 ]
Clerico, Aldo [3 ]
Federico, Giovanni [2 ]
机构
[1] CNR, Inst Clin Physiol, Lab Biochem & Mol Biol, Pisa, Italy
[2] Univ Pisa, Unit Pediat Endocrinol & Diabet, Dept Clin & Expt Med, Pisa, Italy
[3] Scuola Super Sant Anna, Inst Life Sci, Pisa, Italy
关键词
C-type natriuretic peptide; Adipogenesis; Endothelial function; mRNA expression; Reactive hyperemia index; AMINO-TERMINAL PROPEPTIDE; CHRONIC HEART-FAILURE; OBESE CHILDREN; RECEPTOR EXPRESSION; INSULIN-RESISTANCE; PUBERTAL CHANGES; HUMAN-LEUKOCYTES; HEALTHY; MARKERS; RISK;
D O I
10.1016/j.peptides.2019.170218
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-type natriuretic peptide (CNP) is an endogenous adipogenesis regulator whose plasma levels in childhood are known, while no data are available on its expression. Our aim was to evaluate both CNP plasma levels and CNP system expression in whole blood obtained from normal-weight (N, n = 24) and obese (O, n = 16) adolescents (age:13.5 +/- 0.4 years). Endothelial function was assessed measuring reactive hyperemia index (RHI). CNP plasma levels, evaluated with specific RIA, resulted significantly lower in O than in N (6.1 +/- 0.8 vs.15.2 +/- 1.3 pg/mL; p < 0.0001), while CNP/NPR-B/NPR-C mRNA, measured by Real-Time PCR, resulted similar in N (4.1 +/- 1.7; 5.0 +/- 1.6; 2.2 +/- 0.9) and in O (4.3 +/- 1.6; 3.5 +/- 1.1; 2.3 +/- 0.8). RHI was significantly lower in O than in N (1.4 +/- 0.08 vs.2.1 +/- 0.04, p < 0.0001). Dividing all subjects according to the RHI median value, irrespective of the presence or absence of obesity (Group 1 > 1.9, n = 23, Group 2 < 1.9, n = 17), CNP plasma concentrations resulted significantly (p = 0.014) higher in Group 1 (14.6 +/- 1.6) than in Group 2 (7.5 +/- 1.0), showing a significant correlation with RHI (p = 0.0026), while CNP mRNA expression was, surprisingly, higher in Group 2 (7.0 +/- 2.3) than in Group 1 (1.8 +/- 0.4; p = 0.02). NPR-B mRNA resulted similar in both Groups (4.3 +/- 1.6; 4.7 +/- 1.3) and NPR-C significantly higher in Group 2 (p = 0.02). Our data suggest different trends between CNP plasma levels and expression, assessed for the first time in whole blood, that could reflect changes occurring both at CNP transcriptional level in activated leukocytes due to inflammation, and at circulating levels, due to CNP paracrine/autocrine activities. This could represent an interesting area for new therapies able to modulate endothelial dysfunction.
引用
收藏
页数:6
相关论文
共 44 条
  • [11] C-type natriuretic peptide plasma levels are reduced in obese adolescents
    Del Ry, S.
    Cabiati, M.
    Bianchi, V.
    Storti, S.
    Caselli, C.
    Prescimone, T.
    Clerico, A.
    Saggese, G.
    Giannessi, D.
    Federico, G.
    [J]. PEPTIDES, 2013, 50 : 50 - 54
  • [12] C-type natriuretic peptide: A new cardiac mediator
    Del Ry, S.
    [J]. PEPTIDES, 2013, 40 : 93 - 98
  • [13] Plasma C-type natriuretic peptide levels in healthy children
    Del Ry, S.
    Cantinotti, M.
    Cabiati, M.
    Caselli, C.
    Storti, S.
    Prescimone, T.
    Murzi, B.
    Clerico, A.
    Giannessi, D.
    [J]. PEPTIDES, 2012, 33 (01) : 83 - 86
  • [14] C-type natriuretic peptide is closely associated to obesity in Caucasian adolescents
    Del Ry, Silvia
    Cabiati, Manuela
    Bianchi, Vanessa
    Caponi, Laura
    Maltinti, Maristella
    Caselli, Chiara
    Kozakova, Michaela
    Palombo, Carlo
    Morizzo, Carmela
    Marchetti, Sara
    Randazzo, Emioli
    Clerico, Aldo
    Federico, Giovanni
    [J]. CLINICA CHIMICA ACTA, 2016, 460 : 172 - 177
  • [15] Recent advances on natriuretic peptide system: New promising therapeutic targets for the treatment of heart failure
    Del Ry, Silvia
    Cabiati, Manuela
    Clerico, Aldo
    [J]. PHARMACOLOGICAL RESEARCH, 2013, 76 : 190 - 198
  • [16] Della Latta Veronica, 2015, Recenti Progressi in Medicina, V106, P131, DOI 10.1701/1806.19704
  • [17] Increased carotid intima-media thickness in pre-pubertal children with constitutional leanness and severe obesity: the speculative role of insulin sensitivity, oxidant status, and chronic inflammation
    Giannini, C.
    de Giorgis, T.
    Scarinci, A.
    Cataldo, I.
    Marcovecchio, M. L.
    Chiarelli, F.
    Mohn, A.
    [J]. EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2009, 161 (01) : 73 - 80
  • [18] Metabolic, inflammatory, endothelial and haemostatic markers in a group of Italian obese children and adolescents
    Giordano, Paola
    Del Vecchio, Giovanni Carlo
    Cecinati, Valerio
    Delvecchio, Maurizio
    Altomare, Maria
    De Palma, Fabrizia
    De Mattia, Domenico
    Cavallo, Luciano
    Faienza, Maria Felicia
    [J]. EUROPEAN JOURNAL OF PEDIATRICS, 2011, 170 (07) : 845 - 850
  • [19] Inflammatory and non-invasive vascular markers: The multimarker approach for risk stratification in coronary artery disease
    Ikonomidis, Ignatios
    Stamatelopoulos, Kimon
    Lekakis, John
    Vamvakou, Georgia D.
    Kremastinos, Dimitrios Th
    [J]. ATHEROSCLEROSIS, 2008, 199 (01) : 3 - 11
  • [20] Endothelial dysfunction is related to insulin resistance and inflammatory biomarker levels in obese prepubertal children
    Jimenez, Miguel Valle
    Estepa, Rosario Martos
    Camacho, Rosario M' Morales
    Estrada, Ramon Canete
    Luna, Felix Gascon
    Guitarte, Francisco Bermudo
    [J]. EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2007, 156 (04) : 497 - 502