Species-specific PCR assay for the detection of Babesia odocoilei

被引：1
作者：
Burgess, Hilary J. [1 ]
Lockerbie, Betty P. [1 ]
Ayalew, Lisanework E. [1 ]
Dibernardo, Antonia [3 ]
Hrazdilova, Kristyna [4 ]
Modry, David [4 ,5 ,6 ]
Bollinger, Trent K. [1 ,2 ]
机构：
[1] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK, Canada
[2] Univ Saskatchewan, Western Coll Vet Med, Canadian Wildlife Hlth Cooperat, Saskatoon, SK, Canada
[3] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada
[4] Univ Vet & Pharmaceut Sci Brno, CEITEC VFU, Brno, Czech Republic
[5] Masaryk Univ, Fac Sci, Dept Bot & Zool, Brno, Czech Republic
[6] Czech Acad Sci, Biol Ctr, Inst Parasitol, Ceske Budejovice, Czech Republic
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2021年 / 33卷 / 06期
关键词：
Babesia odocoilei; cervids; PCR; specificity; WHITE-TAILED DEER; RIBOSOMAL-RNA; ELK; INFECTION; DIAGNOSIS; GENE;
D O I：
10.1177/10406387211032927
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
引用
收藏
页码：1188 / 1192
页数：5
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