Influence of DNA extraction methods on relative telomere length measurements and its impact on epidemiological studies

被引：41

作者：

Raschenberger, Julia ^{[1
]}

Lamina, Claudia ^{[1
]}

Haun, Margot ^{[1
]}

Kollerits, Barbara ^{[1
]}

Coassin, Stefan ^{[1
]}

Boes, Eva ^{[1
]}

Kedenko, Ludmilla ^{[2
]}

Kottgen, Anna ^{[3
]}

Kronenberg, Florian ^{[1
]}

机构：

[1] Med Univ Innsbruck, Dept Med Genet Mol & Clin Pharmacol, Div Genet Epidemiol, A-6020 Innsbruck, Austria

[2] Paracelsus Med Univ, Salzburger Landeskliniken, Dept Internal Med 1, Salzburg, Austria

[3] Univ Freiburg, Fac Med, Med Ctr, Inst Med Biometry & Stat,Div Genet Epidemiol, Hugstetter Str 55, D-79106 Freiburg, Germany

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

CHRONIC KIDNEY-DISEASE; CARDIOVASCULAR-DISEASE; QUANTITATIVE PCR; HEART-DISEASE; ASSOCIATION; RISK; SMOKING; CANCER; BIAS;

D O I：

10.1038/srep25398

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Measurement of telomere length is widely used in epidemiologic studies. Insufficient standardization of the measurements processes has, however, complicated the comparison of results between studies. We aimed to investigate whether DNA extraction methods have an influence on measured values of relative telomere length (RTL) and whether this has consequences for epidemiological studies. We performed four experiments with RTL measurement in quadruplicate by qPCR using DNA extracted with different methods: 1) a standardized validation experiment including three extraction methods (magnetic-particle-method EZ1, salting-out-method INV, phenol-chloroform-isoamyl-alcohol PCI) each in the same 20 samples demonstrated pronounced differences in RTL with lowest values with EZ1 followed by INV and PCI-isolated DNA; 2) a comparison of 307 samples from an epidemiological study showing EZ1-measurements 40% lower than INV-measurements; 3) a matching-approach of two similar non-diseased control groups including 143 pairs of subjects revealed significantly shorter RTL in EZ1 than INV-extracted DNA (0.844 +/- 0.157 vs. 1.357 +/- 0.242); 4) an association analysis of RTL with prevalent cardiovascular disease detected a stronger association with INV than with EZ1-extracted DNA. In summary, DNA extraction methods have a pronounced influence on the measured RTL-values. This might result in spurious or lost associations in epidemiological studies under certain circumstances.

引用

页数：10

共 38 条

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