The effect of Fe-EDDHA and of ascorbic acid on in vitro rooting of the peach rootstock GF-677 explants

被引:24
作者
Antonopoulou, Chrysovalantou
Dimassi, Kortessa [1 ]
Therios, Ioannis
Chatzissavvidis, Christos
Papadakis, Ioannis
机构
[1] Aristotle Univ Thessaloniki, Sch Agr, Dept Hort, Thessaloniki 54124, Greece
[2] Democritus Univ Thrace, Dept Agr & Dev, Orestiada 68200, Greece
关键词
antioxidant; hybrid; iron; micropropagation; prunus; vitamin C;
D O I
10.1007/s11738-007-0067-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The objective of this research was to study the effect of the chelated form of the iron salt of ethylenediamine di-o-hydroxyphenylacetic acid (Fe-EDDHA) (6% Fe) on in vitro rooting of the rootstock GF-677. The iron salt of ethylenediamine tetraacetic acid (Fe-EDTA) (12% Fe) of the MS basic medium was replaced by Fe- EDDHA, which was applied in three concentrations: 93.5, 187.0 and 280 mg l(-1) (5.6, 11.2 and 16.8 mg l(-1) Fe, respectively). For each treatment of Fe- EDDHA, the effect of ascorbic acid added in four concentrations ( 0, 0.1, 1.0 and 10 mg l(-1)) was studied. After 4 weeks of culture, the explants growing on the medium with 280 mg l(-1) Fe- EDDHA gave the best rooting results. Regarding ascorbic acid, no clear stimulating effect on rooting was found.
引用
收藏
页码:559 / 561
页数:3
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