The scanning electron microscope in microbiology and diagnosis of infectious disease

被引：163

作者：

Golding, Christine G. ^{[1
]}

Lamboo, Lindsey L. ^{[1
,2
]}

Beniac, Daniel R. ^{[1
]}

Booth, Timothy F. ^{[1
,2
]}

机构：

[1] Publ Hlth Agcy Canada, Natl Microbiol Lab, Viral Dis Div, Winnipeg, MB, Canada

[2] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

IONIC LIQUID; SAMPLE PREPARATION; SEM; CELLS;

D O I：

10.1038/srep26516

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy (SEM) is less frequently used than transmission electron microscopy for microbes such as viruses or bacteria. Here we describe rapid methods that allow SEM imaging of fully hydrated, unfixed microbes without using conventional sample preparation methods. We demonstrate improved ultrastructural preservation, with greatly reduced dehydration and shrinkage, for specimens including bacteria and viruses such as Ebola virus using infiltration with ionic liquid on conducting filter substrates for SEM.

引用

页数：8

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