Effects of altering histone posttranslational modifications on mitotic chromosome structure and mechanics

被引：22
作者：
Biggs, Ronald [1 ]
Liu, Patrick Z. [1 ]
Stephens, Andrew D. [1 ]
Marko, John F. [1 ,2 ]
机构：
[1] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA
[2] Northwestern Univ, Dept Phys & Astron, Evanston, IL 60208 USA
来源：
MOLECULAR BIOLOGY OF THE CELL | 2019年 / 30卷 / 07期
基金：
美国国家卫生研究院;
关键词：
CHROMATIN; MITOSIS; LOOP; CONDENSATION; GENERATE;
D O I：
10.1091/mbc.E18-09-0592
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
During cell division, chromatin is compacted into mitotic chromosomes to aid faithful segregation of the genome between two daughter cells. Posttranslational modifications (PTMs) of histones alter compaction of interphase chromatin, but it remains poorly understood how these modifications affect mitotic chromosome stiffness and structure. Using micropipette-based force measurements and epigenetic drugs, we probed the influence of canonical histone PTMs that dictate interphase euchromatin (acetylation) and heterochromatin (methylation) on mitotic chromosome stiffness. By measuring chromosome doubling force (the force required to double chromosome length), we find that histone methylation, but not acetylation, contributes to mitotic structure and stiffness. We discuss our findings in the context of chromatin gel modeling of the large-scale organization of mitotic chromosomes.
引用
收藏
页码：820 / 827
页数：8
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