Induction of liver cytochrome P450 1A2 expression by flutamide in rats

被引：5

作者：

Wang, HX

Liu, X

Xu, CJ

Ma, XC

Long, JE

Li, D ^{[1
]}

机构：

[1] Fudan Univ, Sch Basic Med Sci, Inst Mol Virus, Shanghai 200031, Peoples R China

[2] Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 200032, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Mat Med, Inst Biol Sci, State Key Lab Drug Res, Shanghai 200031, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2005年 / 26卷 / 11期

关键词：

flutamide; cytochrome P450 1A2; induction;

D O I：

10.1111/j.1745-7254.2005.00211.x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Aim: To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats. Methods: Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP 1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate. Results: CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity. Conclusion: Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.

引用

页码：1382 / 1386

页数：5

共 28 条

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