Influenza A H1N1 Virus 2009 Synthetic Hemagglutinin and Neuraminidase Peptides for Antibody Detection

被引:10
作者
Avila, Guillermina [1 ]
Cruz-Licea, Veronica [2 ]
Rojas-Espinosa, Karla [1 ]
Bermudez-Alvarez, Yesenia [1 ]
Grostieta, Estefania [1 ]
Romero-Valdovinos, Mirza [3 ]
Martinez-Hernandez, Fernando [3 ]
Vaughan, Gilberto [4 ]
Flisser, Ana [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Av Univ 3000, Ciudad De Mexico, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Med, Dept Salud Publ, Ciudad De Mexico, Mexico
[3] Hosp Gen Dr Manuel Gea Gonzalez, Ciudad De Mexico, Mexico
[4] Univ Anahuac Mexico Norte, Fac Ciencias Salud, Huixquilucan, Estado De Mexic, Mexico
关键词
Diagnosis; ELISA; IgG; IgA; Influenza; Mexico city; Synthetic peptides; LINKED-IMMUNOSORBENT-ASSAY; IMMUNOGLOBULIN-A; MEXICO; SEROPREVALENCE; INFECTION; RESPONSES;
D O I
10.1016/j.arcmed.2020.04.011
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background. Influenza serologic diagnosis is mainly based on hemagglutination inhibition and microneutralization methods, both methods require handling living viruses under an enhanced biosafety level. Aim. The current study was performed for developing an ELISA using synthetic peptides to detect influenza A H1N1 virus 2009 specific antibodies in serum and saliva. Methods. Alignments were made with H1N1 hemagglutinin and neuraminidase (HA and NA, respectively) sequences; only conserved sites were used for antigenicity prediction. Two synthetic peptides were assayed; one of neuraminidase (NA15) and one of hemagglutinin (HA-15) and used in ELISA for detecting IgG and IgA antibodies. A cross-sectional study was performed in three municipalities of Mexico City, using negative samples collected before the 2009 influenza outbreak, samples of people who became ill during the outbreak, and samples of the participants in the epidemiological study with or without symptoms. Results. The determination of serum IgG antibodies with both peptides allowed differentiating between the post outbreak groups with respect to all others. No differences were found in IgA determination in saliva against both peptides. The frequency of positive participants for NA-15 was 9.5 and 8.8% for HA-15 in serum IgG; whereas the frequency of positive participants for NA-15 was 11%, and for HA-15 was 8.6% for saliva IgA. Conclusions. Synthetic peptides of the neuraminidase and hemagglutinin proteins can be used in ELISA for the determination of IgG and IgA antibodies against the influenza A H1N1 virus 2009. (C) 2020 Published by Elsevier Inc. on behalf of IMSS.
引用
收藏
页码:436 / 443
页数:8
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