Genetic insights into the regulatory pathways for continuous flowering in a unique orchid Arundina graminifolia

被引:15
作者
Ahmad, Sagheer [1 ]
Lu, Chuqiao [1 ]
Gao, Jie [1 ]
Ren, Rui [1 ]
Wei, Yonglu [1 ]
Wu, Jieqiu [1 ]
Jin, Jianpeng [1 ]
Zheng, Chuanyuan [1 ]
Zhu, Genfa [1 ]
Yang, Fengxi [1 ]
机构
[1] Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Inno, Guangzhou 510640, Peoples R China
基金
芬兰科学院;
关键词
Arundina graminifolia; Flowering; Hormone signaling; WGCNA; Transcriptome; TRANSCRIPTOME ANALYSIS; GLOBAL TRANSCRIPTOME; FLORAL TRANSITION; GROWTH-REGULATORS; ARABIDOPSIS; AUXIN; PROTEIN; GENOME; FT; IDENTIFICATION;
D O I
10.1186/s12870-021-03350-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background Manipulation of flowering time and frequency of blooming is key to enhancing the ornamental value of orchids. Arundina graminifolia is a unique orchid that flowers year round, although the molecular basis of this flowering pattern remains poorly understood. Results We compared the A. graminifolia transcriptome across tissue types and floral developmental stages to elucidate important genetic regulators of flowering and hormones. Clustering analyses identified modules specific to floral transition and floral morphogenesis, providing a set of candidate regulators for the floral initiation and timing. Among candidate floral homeotic genes, the expression of two FT genes was positively correlated with flower development. Assessment of the endogenous hormone levels and qRT-PCR analysis of 32 pathway-responsive genes supported a role for the regulatory networks in floral bud control in A. graminifolia. Moreover, WGCNA showed that flowering control can be delineated by modules of coexpressed genes; especially, MEgreen presented group of genes specific to flowering. Conclusions Candidate gene selection coupled with hormonal regulators brings a robust source to understand the intricate molecular regulation of flowering in precious orchids.
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页数:13
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