Cation-Induced Stabilization and Denaturation of DNA Origami Nanostructures in Urea and Guanidinium Chloride

被引：33

作者：

Ramakrishnan, Saminathan ^{[1
]}

Krainer, Georg ^{[2
,3
]}

Grundmeier, Guido ^{[1
]}

Schlierf, Michael ^{[2
]}

Keller, Adrian ^{[1
]}

机构：

[1] Paderborn Univ, Tech & Macromol Chem, Warburger Str 100, D-33098 Paderborn, Germany

[2] Tech Univ Dresden, B CUBE Ctr Mol Bioengn, Arnoldstr 18, D-01307 Dresden, Germany

[3] Univ Kaiserslautern, Mol Biophys, Erwin Schrodinger Str 13, D-67663 Kaiserslautern, Germany

来源：

SMALL | 2017年 / 13卷 / 44期

关键词：

denaturation; DNA origami; guanidinium chloride; salting out; single-molecule biophysics; THERMAL-STABILITY; FOLDING DNA; HYDROCHLORIDE; DELIVERY; PAIR;

D O I：

10.1002/smll.201702100

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The stability of DNA origami nanostructures under various environmental conditions constitutes an important issue in numerous applications, including drug delivery, molecular sensing, and single-molecule biophysics. Here, the effect of Na+ and Mg2+ concentrations on DNA origami stability is investigated in the presence of urea and guanidinium chloride (GdmCl), two strong denaturants commonly employed in protein folding studies. While increasing concentrations of both cations stabilize the DNA origami nanostructures against urea denaturation, they are found to promote DNA origami denaturation by GdmCl. These inverse behaviors are rationalized by a salting-out of Gdm(+) to the hydrophobic DNA base stack. The effect of cation-induced DNA origami denaturation by GdmCl deserves consideration in the design of single-molecule studies and may potentially be exploited in future applications such as selective denaturation for purification purposes.

引用

页数：5

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