Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay

被引：59

作者：

Schmoelz, Lisa ^{[1
,2
]}

Wallert, Maria ^{[1
,3
]}

Lorkowski, Stefan ^{[1
,2
]}

机构：

[1] Friedrich Schiller Univ Jena, Inst Nutr, Dept Nutr Biochem & Physiol, Dornbutger Str 25, D-07743 Jena, Germany

[2] Competence Cluster Nutr & Cardiovasc Hlth nutriCA, Halle Jena Leipzig, Germany

[3] Baker Heart & Diabet Inst, 75 Commercial Rd, Melbourne, Vic 3004, Australia

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2017年 / 449卷

关键词：

Griess assay; Nitric oxide; Long-chain metabolites of alpha-tocopherol; alpha-13 '-COOH; Incubation regime;

D O I：

10.1016/j.jim.2017.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Griess assay is used to measure nitric oxide concentrations in liquid solutions after reaction into nitrite. The assay is challenging when applied to cell culture supernatants. During optimization, we focused on the anti-inflammatory potential of test compounds in murine RAW264.7 macrophages. This led to (i) the required inductivity of cells by lipopolysaccharide (LPS) and allowed (ii) the characterization of putative anti-inflammatory test compounds with high sensitivity. The modifications reported here prominently improved resolution and efficiency of the widely used Griess assay and are of broad interest for studies on the pharmacological modulation of macrophages activation. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：68 / 70

页数：3

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