ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses

被引：174

作者：

Hayakawa, Sumio ^{[1
]}

Shiratori, Souichi ^{[1
,2
]}

Yamato, Hiroaki ^{[1
,3
]}

Kameyama, Takeshi ^{[1
]}

Kitatsuji, Chihiro ^{[1
]}

Kashigi, Fumi ^{[1
,7
]}

Goto, Showhey ^{[1
,7
]}

Kameoka, Shoichiro ^{[1
,7
]}

Fujikura, Daisuke ^{[4
]}

Yamada, Taisho ^{[1
]}

Mizutani, Tatsuaki ^{[5
]}

Kazumata, Mika ^{[1
]}

Sato, Maiko ^{[1
]}

Tanaka, Junji ^{[2
]}

Asaka, Masahiro ^{[3
]}

Ohba, Yusuke ^{[5
]}

Miyazaki, Tadaaki ^{[4
]}

Imamura, Masahiro ^{[2
]}

Takaoka, Akinori ^{[1
,6
]}

机构：

[1] Hokkaido Univ, Div Signaling Canc & Immunol, Inst Med Genet, Sapporo, Hokkaido, Japan

[2] Hokkaido Univ, Dept Hematol & Oncol, Grad Sch Med, Sapporo, Hokkaido, Japan

[3] Hokkaido Univ, Dept Gastroenterol, Grad Sch Med, Sapporo, Hokkaido, Japan

[4] Hokkaido Univ, Dept Bioresources, Res Ctr Zoonosis Control, Sapporo, Hokkaido, Japan

[5] Hokkaido Univ, Lab Pathophysiol & Signal Transduct, Grad Sch Med, Sapporo, Hokkaido, Japan

[6] Hokkaido Univ, Res Ctr Infect Associated Canc, Inst Med Genet, Sapporo, Hokkaido, Japan

[7] Hokkaido Univ, Dept Chem, Grad Sch Sci, Sapporo, Hokkaido, Japan

来源：

NATURE IMMUNOLOGY | 2011年 / 12卷 / 01期

关键词：

NF-KAPPA-B; POLY(ADP-RIBOSE) POLYMERASE; CYTOSOLIC DNA; 5'-TRIPHOSPHATE RNA; UBIQUITIN LIGASE; ADAPTER PROTEIN; INNATE IMMUNITY; RECOGNITION; VIRUS; SENSOR;

D O I：

10.1038/ni.1963

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The poly(ADP-ribose) polymerases (PARPs) participate in many biological and pathological processes. Here we report that the PARP-13 shorter isoform (ZAPS), rather than the full-length protein (ZAP), was selectively induced by 5'-triphosphate-modified RNA (3pRNA) and functioned as a potent stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I. ZAPS associated with RIG-I to promote the oligomerization and ATPase activity of RIG-I, which led to robust activation of IRF3 and NF-kappa B transcription factors. Disruption of the gene encoding ZAPS resulted in impaired induction of interferon-alpha (IFN-alpha), IFN-beta and other cytokines after viral infection. These results indicate that ZAPS is a key regulator of RIG-I signaling during the innate antiviral immune response, which suggests its possible use as a therapeutic target for viral control.

引用

页码：37 / U56

页数：9

共 55 条

[1] RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate [J].

Ablasser, Andrea ;

Bauernfeind, Franz ;

Hartmann, Gunther ;

Latz, Eicke ;

Fitzgerald, Katherine A. ;

Hornung, Veit .

NATURE IMMUNOLOGY, 2009, 10 (10) :1065-U40

[2] B-aggressive lymphoma family proteins have unique domains that modulate transcription and exhibit poly(ADP-ribose) polymerase activity [J].

Aguiar, RCT ;

Takeyama, K ;

He, CY ;

Kreinbrink, K ;

Shipp, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (40) :33756-33765

[3] Negative regulation of the RIG-I signaling by the ubiquitin ligase RNF125 [J].

Arimoto, Kei-ichiro ;

Takahashi, Hitoshi ;

Hishiki, Takayuki ;

Konishi, Hicleyuki ;

Fujita, Takashi ;

Shimotohno, Kunitada .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (18) :7500-7505

[4] Antiviral defense: RIG-Ing the immune system to STING [J].

Bowzard, J. Bradford ;

Ranjan, Priya ;

Sambhara, Suryaprakash ;

Fujita, Takashi .

CYTOKINE & GROWTH FACTOR REVIEWS, 2009, 20 (01) :1-5

[5] p72 DEAD box RNA helicase is required for optimal function of the zinc-finger antiviral protein [J].

Chen, Guifang ;

Guo, Xuemin ;

Lv, Fengxiang ;

Xu, Yihui ;

Gao, Guangxia .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (11) :4352-4357

[6] RNA Polymerase III Detects Cytosolic DNA and Induces Type I Interferons through the RIG-I Pathway [J].

Chiu, Yu-Hsin ;

MacMillan, John B. ;

Chen, Zhijian J. .

CELL, 2009, 138 (03) :576-591

[7] NLRC5 Negatively Regulates the NF-κB and Type I Interferon Signaling Pathways [J].

Cui, Jun ;

Zhu, Liang ;

Xia, Xiaojun ;

Wang, Helen Y. ;

Legras, Xavier ;

Hong, Jun ;

Ji, Jiabing ;

Shen, Pingping ;

Zheng, Shu ;

Chen, Zhijian J. ;

Wang, Rong-Fu .

CELL, 2010, 141 (03) :483-496

[8] The C-terminal regulatory domain is the RNA 5′-triphosphate sensor of RIG-I [J].

Cui, Sheng ;

Eisenaecher, Katharina ;

Kirchhofer, Axel ;

Brzozka, Krzysztof ;

Lammens, Alfred ;

Lammens, Katja ;

Fujita, Takashi ;

Conzelmann, Karl-Klaus ;

Krug, Anne ;

Hopfner, Karl-Peter .

MOLECULAR CELL, 2008, 29 (02) :169-179

[9] Targeted mutagenesis in zebrafish using customized zinc-finger nucleases [J].

Foley, Jonathan E. ;

Maeder, Morgan L. ;

Pearlberg, Joseph ;

Joung, J. Keith ;

Peterson, Randall T. ;

Yeh, Jing-Ruey J. .

NATURE PROTOCOLS, 2009, 4 (12) :1855-1868

[10] The tumour suppressor CYLD is a negative regulator of RIG-I-mediated antiviral response [J].

Friedman, Constantin S. ;

O'Donnell, Marie Anne ;

Legarda-Addison, Diana ;

Ng, Aylwin ;

Cardenas, Washington B. ;

Yount, Jacob S. ;

Moran, Thomas M. ;

Basler, Christopher F. ;

Komuro, Akihiko ;

Horvath, Curt M. ;

Xavier, Ramnik ;

Ting, Adrian T. .

EMBO REPORTS, 2008, 9 (09) :930-936

← 1 2 3 4 5 6 →