The copepod Tigriopus japonicus genomic DNA information (574 Mb) and molecular anatomy

被引：28
作者：
Lee, Jae-Seong [1 ]
Rhee, Jae-Sung [2 ]
Kim, Ryeo-Ok [1 ]
Hwang, Dae-Sik [2 ]
Han, Jeonghoon [2 ]
Choi, Beom-Soon [2 ]
Park, Gyung Soo [3 ]
Kim, Il-Chan [4 ]
Park, Heum Gi [5 ]
Lee, Young-Mi [6 ]
机构：
[1] Hanyang Univ, Dept Chem, Coll Nat Sci, Seoul 133791, South Korea
[2] Hanyang Univ, Dept Mol & Environm Biosci, Grad Sch, Seoul 133791, South Korea
[3] Anyang Univ, Dept Marine Biotechnol, Coll Liberal Arts & Sci, Ganghwa 417833, South Korea
[4] Korea Ocean Res & Dev Inst, Polar BioCtr, Korea Polar Res Inst, Inchon 406840, South Korea
[5] Wonju Natl Univ, Fac Marine Biosci & Technol, Coll Life Sci, Kangnung 210702, South Korea
[6] Sangmyung Univ, Dept Life Sci, Coll Nat Sci, Seoul 110743, South Korea
来源：
MARINE ENVIRONMENTAL RESEARCH | 2010年 / 69卷
关键词：
Copepod; Tigriopus japonicus; GS FLX; GS FLX-Titanium; Genomic DNA; Unigene; GENE-EXPRESSION; GST;
D O I：
10.1016/j.marenvres.2009.12.003
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The intertidal copepod Tigriopus japonicus has been recognized as a promising model species for marine environmental genomics To obtain extensive genomic DNA sequences from this species we sequenced genomic DNA from adult copepods using genomic sequencers GS-FLX and GS-FLX-Titanium and attained 1 914 995 reads (average read length 299 8 bp) including 574 2 Mb of genomic DNA information After subjecting them to assembly we acquired 193 642 contigs (total contigs length 129 7 Mb) and finally were able to obtain 10 894 unigenes (E-value > 0 1 length > 200 bp) containing 33 081 455 bp after a nonredundant (NR) blast search In this paper we summarize the genomic DNA sequences of T japonicus and discuss its potential use in environmental genomics and ecotoxicological studies for uncovering mechanisms of environmental stresses and chemical toxicities to marine crustaceans (C) 2009 Elsevier Ltd All rights reserved
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页码：S21 / S23
页数：3
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