Reducing sugars trigger δ-aminolevulinic dehydratase inactivation:: Evidence of in vitro aspirin prevention

被引：21

作者：

Caballero, FA ^{[1
]}

Gerez, EN ^{[1
]}

Polo, CF ^{[1
]}

Vazquez, ES ^{[1
]}

Batlle, AMD ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, CIPYP, RA-1428 Buenos Aires, DF, Argentina

来源：

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM | 1998年 / 31卷 / 03期

关键词：

glycation; carbohydrates; acetyl salicylic acid; lipid peroxidation; delta-aminolevulinic dehydratase; enzyme inactivation; glycated hemoglobin;

D O I：

10.1016/S0306-3623(98)00016-0

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. The effect of in vitro glycation on delta-aminolevulinic dehydratase (ALA-D) under several experimental conditions was investigated. When preincubated with 500 mM glucose at 37 degrees C for 20 hr, ALA-D was 80% inactivated and glycated hemoglobin levels were increased more than fourfold. 2. Thiobarbituric acid species were not modified during glycation; therefore ALA-D inactivation cannot be attributed to glucose autoxidation. 3. Acetyl salicylic acid was effective in preventing both hemoglobin glycation and ALA-D inactivation by glucose. 4. A method has been developed for measuring protein glycation in vitro, in a crude preparation of red blood cells, which can also he applied to sugars other than glucose. (C) 1998 Elsevier Science Inc.

引用

页码：441 / 445

页数：5

共 24 条

[1] PURIFICATION AND GENERAL PROPERTIES OF DELTA-AMINOLAEVULATE DEHYDRATASE FROM COW LIVER [J].