DDX21 promotes gastric cancer proliferation by regulating cell cycle

被引：43

作者：

Cao, Jiayi ^{[1
]}

Wu, Nan ^{[1
]}

Han, Yuying ^{[1
]}

Hou, Qiuqiu ^{[1
]}

Zhao, Yu ^{[1
]}

Pan, Yanan ^{[1
]}

Xie, Xin ^{[1
]}

Chen, Fulin ^{[1
]}

机构：

[1] Northwest Univ, Fac Life Sci, Lab Tissue Engn, 229 Taibai North Rd, Xian 710069, Shaanxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 505卷 / 04期

基金：

中国国家自然科学基金;

关键词：

DEAD (Asp-Glu-Ala-Asp) box helicase 21 (DDX21); Gastric cancer; Proliferation; Cyclin D1; CDK2; HELICASE; EXPRESSION; IDENTIFICATION; TRANSFORMATION; PROGRESSION; ACTIVATION; ONCOGENE; COMPLEX; GROWTH; NOTCH1;

D O I：

10.1016/j.bbrc.2018.10.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DEAD (Asp-Glu-Ala-Asp) cassette helicase 21 (DDX21) is an ATP-dependent RNA helicase that is overexpressed in various malignancies. There is increasing evidence that DDX21 is involved in carcinogenesis and cancer progression by promoting cell proliferation. However, the functional role of DDX21 in gastric cancer is largely unknown. In this study, we observed that DDX21 was significantly up-regulated in gastric cancer tissues compared to paired adjacent normal tissues. The expression of DDX21 was closely related to the pathological stage of gastric cancer. In vitro and in vivo studies had shown that knockdown of DDX21 inhibited gastric cancer cell proliferation, colony formation, CO cell cycle transition and xenograft growth, while ectopic expression of DDX21 promoted these cell functions. Mechanically, DDX21 induced gastric cancer cell growth by up-regulating levels of Cyclin D1 and CDK2. Taken together, these results revealed a novel role for DDX21 in the proliferation of gastric cancer cells via the Cyclin D1 and CDK2 pathways. Therefore, DDX21 can be used as a therapeutic target for gastric cancer. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：1189 / 1194

页数：6

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