Genetic plasticity in RNA2 is associated with pathogenic diversification of broad bean wilt virus 2

被引：3

作者：

Kim, Myung-Hwi ^{[1
,2
]}

Kwak, Hae-Ryun ^{[3
]}

Choi, Boram ^{[4
,5
]}

Kwon, Sun-Jung ^{[4
,5
]}

Seo, Jang-Kyun ^{[1
,2
,4
,5
]}

机构：

[1] Seoul Natl Univ, Dept Int Agr Technol, Pyeongchang 25354, South Korea

[2] Seoul Natl Univ, Integrated Major Global Smart Farm, Seoul 08826, South Korea

[3] Natl Inst Agr Sci, Crop Protect Div, Rural Dev Adm, Wonju 55365, South Korea

[4] Seoul Natl Univ, Inst Green Bio Sci, Pyeongchang 25354, South Korea

[5] Seoul Natl Univ, Inst Technol, Pyeongchang 25354, South Korea

来源：

VIRUS RESEARCH | 2021年 / 304卷

基金：

新加坡国家研究基金会;

关键词：

Broad bean wilt virus 2; Population diversity; Pathogenicity; Symptomatic variation; SYMPTOM SEVERITY; VARIABILITY; DETERMINANT; PROTEIN;

D O I：

10.1016/j.virusres.2021.198533

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Broad bean wilt virus 2 (BBWV2) is an evolutionarily successful RNA virus with an extensive host range and worldwide distribution that causes severe damage to crops. While numerous BBWV2 isolates from various plant species have been identified and their genome sequences determined, little information is available on the virulence and symptomatic characteristics corresponding to the genomic sequences. In this study, we provide integrated information on the molecular and pathogenic characteristics of three genetically distant BBWV2 isolates: BBWV2-PC, -LS2, and P3 obtained from Gentiana scabra, Leonurus sibiricus, and Pisum sativum, respectively. Phylogenetic and diversity analyses of the BBWV2 population included 42 isolates from various host species and revealed that RNA2 has higher genetic plasticity than RNA1 and may have evolved under hostimposed constraints. In addition, we generated an infectious cDNA clone of BBWV2-PC RNA2 (pBBWV2-PCR2). Pseudo-recombination analysis of pBBWV2-PC-R2 further demonstrated that RNA2 determines the pathogenic characteristics of the PC isolate.

引用

页数：5