Co-Quenching Effect between Lanthanum Metal-Organic Frameworks Luminophore and Crystal Violet for Enhanced Electrochemiluminescence Gene Detection

Exploring new electrochemiluminescence (ECL) luminophores to construct high-efficiency sensing systems is always a hot direction for developing ECL sensors. Compared with other luminophores, metal-organic frameworks (MOFs) exhibit high mass transfer ability for accelerating the reactivity in its pore channels, which is conducive to improving the performance of ECL sensors. In this work, La3+-BTC MOFs (LaMOFs) are prepared as the highly active reactor and novel ECL luminophore. On this basis, a novel co-quenching effect mechanism is proposed based on double-stranded DNA (dsDNA) triggered cooperation between LaMOFs and crystal violet (CV) molecules. Under the confined pore channels of LaMOFs, CV can play an important role as the photon-acceptor due to the matched absorption spectrum with the ECL spectrum of LaMOFs, and the electron-acceptor on account of its lowest unoccupied molecular orbital level. Based on the proposed co-quenching effect mechanism, a constructed ECL gene sensor shows good assay performance toward p53 gene in the detection range of 1 pm to 100 nm with a detection limit of 0.33 pm. The co-quenching effect integrating LaMOFs with CV is expected to be a versatile approach in the construction of ECL gene sensor, which has good prospect in expanding the application range of ECL technology.