Generation of Monoclonal Antibodies Against Recombinant AtSIZ1

被引：1

作者：

Kim, Kangchang ^{[2
]}

Bae, Suyoung ^{[1
]}

Hong, Jaewoo ^{[1
]}

Choi, Jida ^{[1
]}

Ryoo, Soyoon ^{[1
]}

Jhun, Hyunjhung ^{[1
]}

Lee, Siyoung ^{[3
]}

Her, Erk ^{[3
]}

Hong, Kwangwon ^{[1
]}

Kim, Soohyun ^{[1
]}

机构：

[1] Konkuk Univ, Lab Cytokine Immunol, Inst Biomed Sci & Technol, Med Immunol Ctr, Seoul 143701, South Korea

[2] Gyeongsang Natl Univ, PMBBRC, EB NCRC, Div Appl Life Sci,Brain Korea Program 21, Jinju, South Korea

[3] Konkuk Univ, Coll Med, Dept Immunol, Seoul 143701, South Korea

来源：

HYBRIDOMA | 2010年 / 29卷 / 04期

关键词：

SUMO E3 LIGASE; SALICYLIC-ACID; POSTTRANSLATIONAL MODIFICATIONS; POLYPEPTIDE TAGS; MODIFIER SUMO; ARABIDOPSIS; UBIQUITIN; PROTEIN; SUMOYLATION; TRANSCRIPTION;

D O I：

10.1089/hyb.2010.0002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Post-translational modifications of target proteins by small ubiquitin-like modifier (SUMO) proteins modulate many cellular processes in yeast and animals. Here we present the development of monoclonal antibodies (MAb) and polyclonal antibodies (PAb) against Arabidopsis SIZ1 (AtSIZ1) protein with high specificity. Mice were immunized with recombinant AtSIZ1 protein for generating monoclonal antibodies via the classic hybridoma production technique. Anti-AtSIZ1 MAb and PAb were able to detect endogenous AtSIZ1 in Arabidopsis wild type and its complementary line formed by transforming C-siz1-2 mutant with construct containing the AtSIZ1 gene under the control of the native promoter, but not the siz1-2 deletion mutant. These results show that these anti-AtSIZ MAbs are highly sensitive to detect endogenous AtSIZ1 and can be used for immuno-blotting and other experimental methods. The new anti-AtSIZ1 MAbs will be essential tools used to investigate the role of AtSIZ1 in plant developmental biology.

引用

页码：333 / 340

页数：8

共 37 条

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