Effect of shear stress on growth, adhesion and biofilm formation of Pseudomonas aeruginosa with antibiotic-induced morphological changes

被引:24
作者
Fonseca, A. P.
Sousa, J. C.
机构
[1] Univ Porto, Fac Med, Dept Microbiol, P-4100 Oporto, Portugal
[2] Univ Porto, Inst Mol Pathol & Immunol, IRATIMUP, P-4100 Oporto, Portugal
[3] Univ Fernando Pessoa, Fac Hlth Sci, Dept Microbiol, Oporto, Portugal
关键词
Pseudomonas aeruginosa; adhesion; biofilm; dynamic model; morphology; antibiotic;
D O I
10.1016/j.ijantimicag.2007.04.011
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The aim of this study was to investigate the effect of shear stress and antibiotic-induced morphological changes on the growth, adhesion and biofilm formation ability of Pseudomonas aeruginosa. A modified microtitre plate assay was used to quantify adhesion, biofilm formation and planktonic culture density of P. aeruginosa ATCC 27853 under the effect of 0.5 x minimal inhibitory concentrations (MICs) of piperacillin/tazobactam, imipenem and meropenem. Hydrodynamic conditions were achieved by orbital shaking at 250 rpm with the presence of a glass bead in each microtitre well. These conditions decreased adhesion and biofilm formation abilities, increased planktonic culture density over 1 h and decreased planktonic culture density over 16 h for bacteria with antibiotic-induced morphological changes in comparison with static conditions. Our results demonstrate the importance of using a high-throughput dynamic model to assess the adhesion and biofilm formation behaviour of R aeruginosa with antibiotic-induced morphological changes and suggest the possible use of sub-MIC antibiotics in clinical applications to prevent infections acquired by haematogenous spread. This dynamic model provides a better simulation of in vivo conditions of adhesion and biofilm formation of R aeruginosa with altered morphologies induced by beta-lactam antibiotics. (C) 2007 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:236 / 241
页数:6
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