Within day and between day variation of the in vitro under agarose chemotaxis assay in bovine

被引:5
作者
vanWerven, T [1 ]
vandenBroek, J [1 ]
NoordhuizenStassen, EN [1 ]
Daemen, AJJM [1 ]
Schukken, YH [1 ]
Brand, A [1 ]
机构
[1] UNIV UTRECHT, CTR BIOSTAT, NL-3508 TD UTRECHT, NETHERLANDS
关键词
under agarose; chemotaxis; day-to-day variation; bovine;
D O I
10.1016/S0165-2427(96)05723-6
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The present study was conducted to determine within day variation (experiment I) and between day variation (experiment II) of the in vitro under agarose chemotaxis assay; Further, results from experiment II were used to estimate a more stable immunological parameter for the chemotactic activity. In experiment I, blood samples of eight cows were taken every 4 h starting at 0800 during a 24 h period. This procedure was replicated on three different days with peripheral white blood cells of lactating bovine. Chemotactic differential showed variation within a day. The differences between samplings were not constant over the days, but varied randomly from day to day. In experiment II, 12 cows were followed for 8 consecutive days and blood samples for chemotaxis assay were taken once a day at 0730. Differences between the days were significant. With a conditional auto regression model of the first order adjusted least squares means of each cow were estimated over the 8 consecutive days. The chemotactic value of a day was used to estimate the value of the next day. Expanding the model with more previous days did not improve the model. The results of this study indicate that blood samples for chemotaxis should be taken at the same time of the day to control for within day variation. If a sequence of chemotactic values is available we strongly suggest working with adjusted least square means of chemotactic differentials. These adjusted means show less random variation and are a more stable parameter for chemotactic activity.
引用
收藏
页码:83 / 91
页数:9
相关论文
共 20 条
[1]  
[Anonymous], 1993, MODELS REPEATED MEAS
[3]  
CAI TQ, 1994, AM J VET RES, V55, P934
[4]   MODIFIED METHOD FOR CHEMOTAXIS UNDER AGAROSE [J].
CHENOWETH, DE ;
ROWE, JG ;
HUGLI, TE .
JOURNAL OF IMMUNOLOGICAL METHODS, 1979, 25 (04) :337-353
[6]  
JAIN NC, 1993, ESSENTIALS VET HEMAT
[7]   PERIPARTURIENT HYPOCALCEMIA IN COWS - EFFECTS ON PERIPHERAL-BLOOD NEUTROPHIL AND LYMPHOCYTE FUNCTION [J].
KEHRLI, ME ;
GOFF, JP .
JOURNAL OF DAIRY SCIENCE, 1989, 72 (05) :1188-1196
[8]   SEVERITY OF EXPERIMENTAL ESCHERICHIA-COLI MASTITIS IN KETONEMIC AND NONKETONEMIC DAIRY-COWS [J].
KREMER, WDJ ;
NOORDHUIZENSTASSEN, EN ;
GROMMERS, FJ ;
SCHUKKEN, YH ;
HEERINGA, R ;
BRAND, A ;
BURVENICH, C .
JOURNAL OF DAIRY SCIENCE, 1993, 76 (11) :3428-3436
[9]   PREINFECTION CHEMOTACTIC RESPONSE OF BLOOD POLYMORPHONUCLEAR LEUKOCYTES TO PREDICT SEVERITY OF ESCHERICHIA-COLI MASTITIS [J].
KREMER, WDJ ;
NOORDHUIZENSTASSEN, EN ;
GROMMERS, FJ ;
DAEMEN, AJJM ;
HENRICKS, PAJ ;
BRAND, A ;
BURVENICH, C .
JOURNAL OF DAIRY SCIENCE, 1993, 76 (06) :1568-1574
[10]   VARIABILITY OF THE BLOOD COMPOSITION OF DAIRY-COWS IN RELATION TO TIME OF DAY [J].
MANSTON, R ;
ROWLANDS, GJ ;
LITTLE, W ;
COLLIS, KA .
JOURNAL OF AGRICULTURAL SCIENCE, 1981, 96 (JUN) :593-598