The influence of photodynamic therapy (PDT) with δ-aminolevulinic acid (ALA) on J-774A.1 macrophage cell line

Introduction. The whole mechanism of the cellular level of tumor destruction by photodynamic therapy (PDT) is still unknown. Despite necrotic and apoptotic ways of cell death, there is a variety of events leading to and magnifying the inactivation of tumor cells. Material and methods. J-774A.1 were incubated with delta-aminolevulinic acid (ALA) at different concentrations (125, 250, 500, 1000 M) and then irradiated with VIS (400 - 750 nm) at the dose of 5,10 and 30 J/cm(2) delivered from the incoherent light source. The effects of the application of ALA-PDT were evaluated on the basis of cell viability, nitric oxide (NO), tumor necrosis factor alpha- (TNF-alpha) and interleukin- 1 beta (IL- 1 beta) produced by the J-774A.1 cells. Results. The cell viability (assessed using MTT test) was comparable with control group at 5,10 and 30 J/cm2. At these doses of energy using different concentrations of ALA we have observed that at the higher energy doses, the greater increase of TNF-a release, lowering of the level of IL-1 beta production and decrease of NO release were observed. There was also observed the dependence of the secretional activity, of the cells on the ALA concentrations. Conclusion.. The cell viability and production of cytokines depended on ALA concentrations and energy doses of the light. The higher some cytokines' release after PDT could be an additional factor for the complete eradication of tumor.