Cactus (Opuntia ficus-indica) cladodes prevent oxidative damage induced by the mycotoxin zearalenone in Balb/C mice

Zearalenone (ZEN) is one of the most widely distributed fusarial mycotoxins which is encountered at high incidence in many foodstuffs. ZEN was associated with different reproductive disorders in animals. Several in vivo studies have shown that ZEN is hepatotoxic, haematotoxic and causes several alterations of immunological parameters. Furthermore, evidence of its cytotoxicity and genotoxicity has recently emerged from several reports. The aim of the current study was (i) to find Out whether oxidative stress could be relevant for ZEN induced toxicity in vivo using Balb/c mice and (ii) to evaluate the safety and efficacy of cactus cladodes Opuntia ficus to prevent the deleterious effects of ZEN. To this end, the effect of a single dose of ZEN (40 mg/kg b.w.) alone and with extract of cactus cladodes (25, 50 and 100 mg/kg b.w.) oil the induction of oxidative stress was monitored in kidney and liver by measuring the MDA level, the protein carbonyls generation, the catalase activity and the expression of the heat shock proteins (Hsp). Our results clearly showed that ZEN induced significant alterations in all tested oxidative stress markers. Oxidative damage seems to be a key determinant of ZEN induced toxicity in both liver and kidney of Balb/c mice. The combined treatment of ZEN with the lowest tested dose of cactus extracts (25 mg/kg b.w.) showed a total reduction of ZEN induced oxidative damage for all tested markers. It could be concluded that cactus cladodes extract was effective in the protection against ZEN hazards. This could be relevant, particularly with the emergent demand for natural products which may counteract the detrimental effects of oxidative stress and therefore prevent multiple human diseases. (c) 2008 Elsevier Ltd. All rights reserved.