In-vitro anti-urolithiatic activity and simultaneous HPTLC quantification of berberine and palmatine in standardized extract of Thalictrum foliolosum DC: Root

Thalictrum foliolosum DC. (Ranunculaceae) is a perennial plant distributed worldwide. In India it is used as a diuretic, anti-periodic, febrifuge, jaundice and in rheumatism. The present study was aimed to evaluate in-vitro anti-urolithiatic, anti-oxidant, anti-inflammatory activity along with concurrent quantification of berberine and palmatine through high performance thin layer chromatography (HPTLC). Anti-urolithiatic potential was evaluated via a number of different steps viz. nucleation, aggregation, whole urine assays and through kinetic study. The IC50 value of nucleation and aggregation was found to be 114.314 and 67.625 mu g/mL. Anti-oxidant activity was evaluated by DPPH and total antioxidant capacity with IC50 value 54.96 mu g/mL and 11.35 mg/g anti-oxidant capacity. Anti-inflammatory potential was evaluated through inhibition of protein denaturation and antiproteinase activity, the IC50 values were 94.61 and 60.51 mu g/mL. Separation of berberine and palmatine was done on an aluminum TLC plate precoated with silica gel 60 GF(254) ( 10 x 10 cm), with mobile phase containing n-propanolwater-formic acid (90-10-1 v/v). Berberine and palmatine were identified at R-f 0.44 +/- 0.05 and 0.29 +/- 0.06. Densitometric analysis was carried out at 350 nm for both the biomarkers in absorbancereflectance mode. Berberine and palmatine concentrations were found to be 1.155 +/- 0.035 and 0.111 +/- 0.008 in plant on% dry weight basis. The result indicates that T. foliolosum has significant antioxidant and anti-inflammatory activity, and it is a potential candidate for phytotherapy against urolithiasis because of its ability to inhibit CaOx crystallization. Quantification of biomarkers will aid in quality standardization of raw material. (C) 2022 SAAB. Published by Elsevier B.V. All rights reserved.