Structural characterization of the polar lipids of Clostridium novyi NT. Further evidence for a novel anaerobic biosynthetic pathway to plasmalogens

被引：26

作者：

Guan, Ziqiang ^{[2
]}

Johnston, Norah C. ^{[1
]}

Aygun-Sunar, Semra ^{[3
]}

Daldal, Fevzi ^{[3
]}

Raetz, Christian R. H. ^{[2
]}

Goldfine, Howard ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA

[2] Duke Univ, Dept Biochem, Med Ctr, Durham, NC 27710 USA

[3] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2011年 / 1811卷 / 03期

关键词：

Mass spectrometry; Plasmalogen; Phospholipid; Aminoacyl-phosphatidylglycerol; BUTYRICUM; PHOSPHOLIPIDS; GLYCEROL; BACTERIA; THERAPY; ACETAL;

D O I：

10.1016/j.bbalip.2010.12.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study of the polar lipids of Clostridium novyi NT has revealed the presence of phosphatidylethanolamine (PE) and cardiolipin as major phospholipids with smaller amounts of phosphatidylglycerol (PG), lysyl-PG and alanyl-PG. Other minor phospholipids included phosphatidic acid, CDP-diacylglycerol, phosphatidylserine (PS) and phosphatidylthreonine (PT). PE, PG and amino acyl PG were present in both the diacyl and alk-1'-enyl acyl (plasmalogen) forms and cardiolipin plasmalogens were found to contain one or two alk-1'-enyl chains. In contrast, the precursor lipids phosphatidic acid, CDP-diacylglycerol and PS were present almost exclusively as diacyl phospholipids. These findings are consistent with the hypothesis that plasmalogens are formed from diacylated phospholipids at a late stage of phospholipid formation in Clostridium species. This novel pathway contrasts with the route in animals in which a saturated ether bond is formed at an early stage of plasmalogen biosynthesis and the alk-1-enyl bond is formed by an aerobic mechanism. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：186 / 193

页数：8

共 26 条

[1] Bacteriolytic therapy can generate a potent immune response against experimental tumors [J].

Agrawal, N ;

Bettegowda, C ;

Cheong, I ;

Geschwind, JF ;

Drake, CG ;

Hipkiss, EL ;

Tatsumi, M ;

Dang, LH ;

Diaz, LA ;

Pomper, M ;

Abusedera, M ;

Wahl, RL ;

Kinzler, KW ;

Zhou, SB ;

Huso, DL ;

Vogelstein, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (42) :15172-15177

[2]

[Anonymous], 1983, ETHER LIPIDS BIOCHEM

[3]

[Anonymous], 1986, Techniques of lipidology: isolation, analysis and identification of lipids

[4]

BAUMANN NA, 1965, J BIOL CHEM, V240, P1559

[5] Overcoming the hypoxic barrier to radiation therapy with anaerobic bacteria [J].

Bettegowda, C ;

Dang, LH ;

Abrams, R ;

Huson, DL ;

Dillehay, L ;

Cheong, I ;

Agrawal, N ;

Borzillary, S ;

McCaffery, JM ;

Watson, EL ;

Lin, KS ;

Bunz, F ;

Baidoo, K ;

Pomper, MG ;

Kinzler, KW ;

Vogelstein, B ;

Zhou, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (25) :15083-15088

[6] The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT [J].

Bettegowda, Chetan ;

Huang, Xin ;

Lin, Jimmy ;

Cheong, Ian ;

Kohli, Manu ;

Szabo, Stephen A. ;

Zhang, Xiaosong ;

Diaz, Luis A., Jr. ;

Velculescu, Victor E. ;

Parmigiani, Giovanni ;

Kinzler, Kenneth W. ;

Vogelstein, Bert ;

Zhou, Shibin .

NATURE BIOTECHNOLOGY, 2006, 24 (12) :1573-1580

[7]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[8] Pharmacologic and toxicologic evaluation of C-novyi-NT spores [J].

Diaz, LA ;

Cheong, I ;

Foss, CA ;

Zhang, XS ;

Peters, BA ;

Agrawal, N ;

Bettegowda, C ;

Karim, B ;

Liu, GS ;

Khan, K ;

Huang, X ;

Kohli, M ;

Dang, LH ;

Hwang, P ;

Vogelstein, A ;

Garrett-Mayer, E ;

Kobrin, B ;

Pomper, M ;

Zhou, SB ;

Kinzler, KW ;

Vogelstein, B ;

Huso, DL .

TOXICOLOGICAL SCIENCES, 2005, 88 (02) :562-575

[9]

Goldfine H., 1972, ETHER LIPIDS CHEM BI, P329

[10]

Goldfine Howard, 2005, P297, DOI 10.1201/9780203489819.pt3

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