A new solid-phase extraction and HPLC method for determination of patulin in apple products and hawthorn juice in China

被引：31
作者：
Zhou, Yuchun [1 ,2 ]
Kong, Weijun [1 ]
Li, Yan [1 ,3 ]
Logrieco, Antonio F. [4 ]
Xu, Jun [2 ]
Yang, Meihua [1 ]
机构：
[1] Chinese Acad Med Sci, Inst Med Plant Dev, Peking Union Med Coll, Beijing 100193, Peoples R China
[2] Jiangxi Univ Tradit Chinese Med, Nanchang, Peoples R China
[3] Jiangsu Univ, Sch Pharm, Zhenjiang, Peoples R China
[4] Inst Sci Food Prod, Bari, Italy
来源：
JOURNAL OF SEPARATION SCIENCE | 2012年 / 35卷 / 5-6期
基金：
美国国家科学基金会;
关键词：
HPLC-DAD; LC-ESI-MS; Patulin; PVPP-F column; SPE; CHROMATOGRAPHIC DETERMINATION; FRUIT;
D O I：
10.1002/jssc.201100919
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new solid-phase extraction (SPE) pretreatment method using a home-made polyvinylpolypyrrolidone-florisil (PVPP-F) column was developed for the analysis of patulin in apple and hawthorn products in China. Fifty samples (25 apple juices, 12 apple jams, and 13 hawthorn juices) were prepared using the new method and then analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) on an Agela Venusil MP C18 reversed-phase column (4.6 mm x 250 mm, 5 mu m). The cleanup results for all samples using home-made PVPP-F column were compared with those obtained using a MycoSep (R) 228 AflaPat column. The correlation coefficient R (0.9998) fulfilled the requirement of linearity for patulin in the concentration range of 2.5250 mu g/kg. The limits of detection (LODs) and quantification (LOQs) of patulin were 3.99 and 9.64 mu g/kg for PVPP-F column, and 3.56 and 8.07 mu g/kg for MycoSep (R) 228 AflaPat column, respectively. Samples were spiked with patulin at levels ranging from 25 to 250 mu g/kg, and recoveries using PVPP-F and MycoSep (R) 228 AflaPat columns were in the range of 81.9100.9% and 86.4103.9%, respectively. Naturally occurring patulin was found in 2 of 25 apple juice samples (8.0%) and 1 of 13 hawthorn juice samples (7.7%) at concentrations ranging from 12.26 to 36.81 mu g/kg. The positive results were further confirmed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS).
引用
收藏
页码：641 / 649
页数：9
相关论文
共 22 条
  • [1] Arbizu M. M., 2009, FOOD CHEM, V113, P420
  • [2] Analysis of patulin in dutch food, an evaluation of a SPE based method
    Boonzaaijer, G
    Bobeldijk, I
    van Osenbruggen, WA
    [J]. FOOD CONTROL, 2005, 16 (07) : 587 - 591
  • [3] Determination of patulin in apple and quince products by GC-MS using 13C5-7 patulin as internal standard
    Cunha, S. C.
    Faria, M. A.
    Fernandes, J. O.
    [J]. FOOD CHEMISTRY, 2009, 115 (01) : 352 - 359
  • [4] A new competitive fluorescence assay for the detection of patulin toxin
    de Champdore, Marcella
    Bazzicalupo, Paolo
    De Napoli, Lorenzo
    Montesarchio, Daniela
    Di Fabio, Giovanni
    Cocozza, Immacolata
    Parracino, Antonietta
    Rossi, Mose'
    D'Auria, Sabato
    [J]. ANALYTICAL CHEMISTRY, 2007, 79 (02) : 751 - 757
  • [5] European Commission Regulation, 2006, OFF J EUR UNION L, V364, P5, DOI DOI 10.2203/DOSE-RESPONSE.06-012.HANEKAMP
  • [6] Determination of patulin in solid and semisolid apple and pear products marketed in Argentina
    Funes, G. J.
    Resnik, S. L.
    [J]. FOOD CONTROL, 2009, 20 (03) : 277 - 280
  • [7] Liquid chromatographic method for the determination of patulin in apple juice using solid-phase extraction
    Gökmen, V
    Acar, J
    Sarioglu, K
    [J]. ANALYTICA CHIMICA ACTA, 2005, 543 (1-2) : 64 - 69
  • [8] Effects of various clarification treatments on patulin, phenolic compound and organic acid compositions of apple juice
    Gökmen, V
    Artik, N
    Acar, J
    Kahraman, N
    Poyrazoglu, E
    [J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2001, 213 (03) : 194 - 199
  • [9] Rapid reversed-phase liquid chromatographic determination of patulin in apple juice
    Gokmen, V
    Acar, J
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 730 (1-2) : 53 - 58
  • [10] Development of liquid chromatography-electrospray mass spectrometry for the determination of patulin in apple juice: Investigation of its contamination levels in Japan
    Ito, R
    Yamazaki, H
    Inoue, K
    Yoshimura, Y
    Kawaguchi, M
    Nakazawa, H
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (25) : 7464 - 7468
123