Double-strand break repair: are Rad51/RecA-DNA joints barriers to DNA replication?

被引：29

作者：

Aguilera, A ^{[1
]}

机构：

[1] Univ Sevilla, Fac Biol, Dept Genet, E-41012 Seville, Spain

来源：

TRENDS IN GENETICS | 2001年 / 17卷 / 06期

关键词：

D O I：

10.1016/S0168-9525(01)02309-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The central step of homologous recombination is the DNA strand exchange reaction catalyzed by bacterial RecA or eukaryotic Rad51. Besides Rad51-mediated synthesis-dependent strand annealing (SDSA), DNA ends can promote replication in Escherichia coli(recombination-dependent replication, RDR) and yeast (break-induced replication, BIR). However, what causes a DNA end to be repaired via SDSA or via BIR/RDR? I propose that Rad51/RecA-DNA plectonemic joints act as barriers to DNA replication and that BIR/RDR is only possible when the DNA polymerase that synthesizes DNA from the invading 3' end does not encounter RecA/Rad51-DNA joints in its path.

引用

页码：318 / 321

页数：4

共 39 条

[1] GENETIC-EVIDENCE FOR DIFFERENT RAD52-DEPENDENT INTRACHROMOSOMAL RECOMBINATION PATHWAYS IN SACCHAROMYCES-CEREVISIAE [J].

AGUILERA, A .

CURRENT GENETICS, 1995, 27 (04) :298-305

[2] DNA-REPLICATION TRIGGERED BY DOUBLE-STRANDED BREAKS IN ESCHERICHIA-COLI - DEPENDENCE ON HOMOLOGOUS RECOMBINATION FUNCTIONS [J].

ASAI, T ;

BATES, DB ;

KOGOMA, T .

CELL, 1994, 78 (06) :1051-1061

[3]

Bai Y, 1999, GENETICS, V153, P1117

[4] A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae [J].

Bai, Y ;

Symington, LS .

GENES & DEVELOPMENT, 1996, 10 (16) :2025-2037

[5] RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates [J].

Bärtsch, S ;

Kang, LE ;

Symington, LS .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (04) :1194-1205

[6] Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro [J].

Baumann, P ;

Benson, FE ;

West, SC .

CELL, 1996, 87 (04) :757-766

[7] Recombinational repair of gaps in DNA is asymmetric in Ustilago maydis and can be explained by a migrating D-loop model [J].

Ferguson, DO ;

Holloman, WK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5419-5424

[8] DNA-SYNTHESIS DEPENDENT ON GENETIC-RECOMBINATION - CHARACTERIZATION OF A REACTION CATALYZED BY PURIFIED BACTERIOPHAGE-T4 PROTEINS [J].

FORMOSA, T ;

ALBERTS, BM .

CELL, 1986, 47 (05) :793-806

[9] TARGETED GENE REPLACEMENT IN DROSOPHILA VIA P-ELEMENT-INDUCED GAP REPAIR [J].

GLOOR, GB ;

NASSIF, NA ;

JOHNSONSCHLITZ, DM ;

PRESTON, CR ;

ENGELS, WR .

SCIENCE, 1991, 253 (5024) :1110-1117

[10] Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases [J].

Holmes, AM ;

Haber, JE .

CELL, 1999, 96 (03) :415-424

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