Selection of primers for specific detection of Clostridium botulinum types B and E neurotoxin genes using PCR method

被引：6

作者：

Alsallami, AA

Kotlowski, R

机构：

[1] Gdansk Tech Univ, Fac Chem, Dept Food Chem & Technol, PL-80952 Gdansk, Poland

[2] Aden Univ, Aden, Yemen

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2001年 / 69卷 / 03期

关键词：

Clostridium botulinum; PCR; primer selection;

D O I：

10.1016/S0168-1605(01)00499-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Improved oligonucleotide primers were designed to flank 370- and 307-bp fragments of the bont genes encoding botulinum neurotoxins types B and E, respectively. Primer specificity was confirmed for reference strains of Clostridium botulinum types B and E for strains representing bacterial species common in food, and for the DNA mixtures of C. botulinum types B and E in the presence of background DNA isolated from cold smoked salmon and ham. The detection limit of template DNAs of C. botulinum types B and E from the DNA mixtures increased from 1 to 0.1 ng by raising annealing temperature from 50 degreesC to 62 degreesC. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：247 / 253

页数：7