A One-Health Genomic Investigation of Gentamicin Resistance in Salmonella from Human and Chicken Sources in Canada, 2014 to 2017

被引：14

作者：

Cox, Graham W. ^{[1
,2
]}

Parmley, E. Jane ^{[3
,4
]}

Avery, Brent P. ^{[3
]}

Irwin, Rebecca J. ^{[3
]}

Reid-Smith, Richard J. ^{[3
]}

Deckert, Anne E. ^{[3
]}

Finley, Rita L. ^{[3
]}

Daignault, Danielle ^{[5
]}

Alexander, David C. ^{[6
]}

Allen, Vanessa ^{[7
]}

El Bailey, Sameh ^{[8
]}

Bekal, Sadjia ^{[9
]}

Chui, Linda ^{[10
,11
]}

German, Greg J. ^{[12
]}

Haldane, David ^{[13
]}

Hoang, Linda ^{[14
]}

Minion, Jessica ^{[15
]}

Zahariadis, George ^{[16
]}

Mulvey, Michael R. ^{[1
,2
]}

Bharat, Amrita ^{[1
,2
]}

机构：

[1] Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada

[2] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada

[3] Publ Hlth Agcy Canada, Ctr Food Borne Environm & Zoonot Infect Dis, Guelph, ON, Canada

[4] Univ Guelph, Ontario Vet Coll, Dept Populat Med, Guelph, ON, Canada

[5] Publ Hlth Agcy Canada, Natl Microbiol Lab, St Hyacinthe, PQ, Canada

[6] Cadham Prov Lab, Winnipeg, MB, Canada

[7] Publ Hlth Ontario Labs, Toronto, ON, Canada

[8] Horizon Hlth Network, St John, NB, Canada

[9] Lab Sante Publ Quebec, Ste Anne De Bellevue, PQ, Canada

[10] Alberta Precis Labs Prov Lab Publ Hlth, Edmonton, AB, Canada

[11] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB, Canada

[12] Queen Elizabeth Hosp, Charlottetown, PE, Canada

[13] Queen Elizabeth 2 Hlth Sci Ctr, Halifax, NS, Canada

[14] British Columbia Ctr Dis Control, Vancouver, BC, Canada

[15] Roy Romanow Prov Lab, Regina, SK, Canada

[16] Newfoundland & Labrador Publ Hlth & Microbiol Lab, St John, NF, Canada

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2021年 / 65卷 / 12期

关键词：

aminoglycosides; antimicrobial resistance; genomics; gentamicin; poultry; whole-genome sequencing; SPECTRUM BETA-LACTAMASES; AMINOGLYCOSIDE ANTIBIOTICS; ANTIMICROBIAL RESISTANCE; ESCHERICHIA-COLI; EPIDEMIOLOGY; VIRULENCE; ENTERICA; GENES;

D O I：

10.1128/AAC.00966-21

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated whether the increased prevalence of gentamicin resistance in Salmonella from human infections was related to a similar increased prevalence in isolates from broiler chickens and whether this increase may have been due to coselection from use of lincomycin-spectinomycin in chickens on farms. Whole-genome sequencing was performed on gentamicin-resistant (Genr) Salmonella isolates from human and chicken sources collected from 2014 to 2017 by the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). We determined the genomic relatedness of strains and characterized resistance genes and plasmids. From 2014 to 2017, 247 isolates of Genr Salmonella were identified by CIPARS: 188 were from humans, and 59 were from chicken sources (26 from live animals on farm and 33 from retail meat). The five most common Genr serovars were Salmonella enterica serovars Heidelberg (n = 93; 31.5%), 4,[5],12:i:- (n = 42; 14.2%), Kentucky (n = 37; 12.5%), Infantis (n = 33; 11.2%), and Typhimurium (n = 23; 7.8%). Phylogenomic analysis revealed that for S. Heidelberg and S. Infantis, there were closely related isolates from human and chicken sources. In both sources, resistance to gentamicin and spectinomycin was most frequently conferred by aac(3)-VIa and ant(3'')-Ia, respectively. Plasmid closure confirmed linkages of gentamicin and spectinomycin resistance genes and revealed instances of similar plasmids from both sources. Gentamicin and spectinomycin resistance genes were linked on the same plasmids, and some plasmids and isolates from humans and chickens were genetically similar, suggesting that the use of lincomycin-spectinomycin in chickens may be selecting for gentamicin-resistant Salmonella in broiler chickens and that these resistant strains may be acquired by humans.

引用

页数：12

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