Identification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies

被引：25

作者：

Longyant, Siwaporn ^{[1
]}

Rukpratanporn, Sombat ^{[2
]}

Chaivisuthangkura, Parin ^{[1
]}

Suksawad, Pornthip ^{[1
]}

Srisuk, Chutima ^{[1
]}

Sithigorngul, Weerawan ^{[1
]}

Piyatiratitivorakul, Somkiat ^{[2
,3
]}

Sithigorngul, Paisarn ^{[1
]}

机构：

[1] Srinakharinwirot Univ, Dept Biol, Fac Sci, Bangkok 10110, Thailand

[2] Chulalongkorn Univ, Ctr Excellence Marine Biotechnol, Natl Ctr Genet Engn & Biotechnol, Bangkok 10330, Thailand

[3] Chulalongkorn Univ, Fac Sci, Dept Marine Sci, Bangkok 10330, Thailand

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2008年 / 98卷 / 01期

关键词：

monoclonal antibody; Penaeus vannamei; Vibrio shilonii; V; parahaemolyticus; vulnificus; vibriosis;

D O I：

10.1016/j.jip.2007.10.013

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Immunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and in hepatopancreas tissues with MAbs VVB158 (specific to V vulnificus) and VPC701 (specific to V parahaemolyticus). Twelve isolates of Vibrio spp. isolated from shrimp tissues were identified with various MAbs by dot blotting, biochemical tests and 16S rRNA gene. The results revealed three groups of V. vulnificus and one group of V. shilonii. All four groups of isolated Vibrio spp. were immunologically and biochemically different. None of the V parahaemolyticus-like bacterium was isolated. The results demonstrated that the mortality in shrimp is accompanied by the presence of Vibrio spp. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：63 / 68

页数：6

共 34 条

[1] Differences in the susceptibility of American white shrimp larval substages (Litopenaeus vannamei) to four Vibrio species [J].