Reversed-phase monoliths prepared by UV polymerization of divinylbenzene

被引：9

作者：

Flook, Kelly ^{[1
]}

Agroskin, Yury ^{[1
]}

Pohl, Chris ^{[1
]}

机构：

[1] Dionex Chem Corp, Sunnyvale, CA USA

来源：

JOURNAL OF SEPARATION SCIENCE | 2011年 / 34卷 / 16-17期

关键词：

Chromatography; Monolith; Protein analysis; UV polymerization; CONTROLLED POROUS PROPERTIES; PHOTOINITIATED POLYMERIZATION; STATIONARY PHASES; STYRENE; PERFORMANCE; SEPARATION; PROTEINS; COLUMNS;

D O I：

10.1002/jssc.201100196

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Many different techniques have been developed to prepare monolithic materials specifically for chromatographic techniques. The two most popular polymerization techniques being thermal or via ultra violet (UV) light. Whereas thermal polymerization is easily employed for a whole variety of monomer and porogen systems, UV polymerization has been limited to methacrylate-based systems, and styrenic systems have been avoided due to their strong absorbance at low wavelengths. By careful consideration of wavelength, initiator and other system components, it was proven that reversed-phase columns for the separation of proteins and peptides can be prepared using divinylbenzene through UV initiation of 2-methyl-4'-(methylthio)-2-morpholinopropiophenone at a wavelength of 350 nm.

引用

页码：2047 / 2053

页数：7

共 22 条

[1] UV-LED photopolymerised monoliths [J].