Circular RNA circ-RELL1 regulates inflammatory response by miR-6873-3p/MyD88/NF-κB axis in endothelial cells

被引：51

作者：

Huang, Hua-shan ^{[1
,2
]}

Huang, Xiao-yan ^{[3
]}

Yu, Hui-zhen ^{[1
,2
]}

Xue, Yan ^{[1
,2
]}

Zhu, Peng-li ^{[1
,2
]}

机构：

[1] Fujian Med Univ, Shengli Clin Med Coll, Fuzhou 350001, Fujian, Peoples R China

[2] Fujian Prov Hosp, Key Lab Geriatr, Fuzhou 350001, Fujian, Peoples R China

[3] Fujian Med Univ, Dept Gastr Surg, Union Hosp, Fuzhou, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 525卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Circ-RELL1; miR-6873-3p; MyD88; NF-kappa Bp65; Inflammatory response;

D O I：

10.1016/j.bbrc.2020.02.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endothelial inflammation is an important contributor to the pathology of atherosclerotic cardiovascular disease (ASCVD). Circular RNAs (circRNAs) function and role in endothelium inflammation still unknown. In our present study, we firstly identified that circ-RELL1 plays a proinflammatory role in ox-LDL-induced HUVECs through high-throughput circRNA microarray assays. Knockdown circ-RELL1 can reduce the expression of ICAM1 and VCAM1 in ox-LDL induced endothelium inflammation. Mechanistically, circ-RELL1 directly bound to miR-6873-3p in cytoplasm. Subsequently miR-6873-3p reduced MyD88 (myeloid differentiation primary response 88) protein expression and alleviated MyD88 medicated NF-kappa B activation. Furthermore, circ-RELL1 can abolish the inhibition of inflammation response by miR-6873-3p. Our findings illustrate a novel regulatory pathway that circ-RELL1 modulate inflammatory response by miR-6873-3p/MyD88/NF-kappa B axis in ox-LDL induced endothelial cells, which provides a potential therapeutic candidate for endothelium inflammation in atherosclerotic cardiovascular disease. (C) 2020 The Author(s). Published by Elsevier Inc.

引用

页码：512 / 519

页数：8