Molecular cloning, expression profiling and functional analysis of a DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Camptotheca acuminata

被引：38

作者：

Yao, Hongyan ^{[1
]}

Gong, Yifu ^{[1
,3
]}

Zuo, Kaijing ^{[1
]}

Ling, Hua ^{[1
]}

Qiu, Chengxiang ^{[1
]}

Zhang, Fei ^{[1
]}

Wang, Yechun ^{[1
]}

Pi, Yan ^{[2
]}

Liu, Xiang ^{[1
]}

Sun, Xiaofen ^{[2
]}

Tang, Kexuan ^{[1
,2
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Sch Life Sci & Technol, Plant Biotechnol Res Ctr,Fudan SJTU Nottingham Pl, Shanghai 200030, Peoples R China

[2] Fudan Univ, Morgan Tan Int Ctr Life Sci, Fudan SJTU Nottingham Plant R&D Ctr, Inst Genet, Shanghai 200433, Peoples R China

[3] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 165卷 / 02期

关键词：

Camptotheca acuminata; Camptothecin biosynthesis; 1-deoxy-D-xylulose 5-phosphate reductoisomerase; methyl jasmonate; rapid amplification of cDNA ends;

D O I：

10.1016/j.jplph.2006.12.001

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

As the second enzyme of the non-mevalonate terpenoid pathway for isopentenyl diphosphate biosynthesis, DXP reductoisomerase (DXR, EC: 1.1.1.267) catalyzes a committed step of the MEP pathway for camptothecin (CPT) biosynthesis. In order to understand more about the role of DXR involved in the CPT biosynthesis at the molecular Level, the full-Length DXR cDNA sequence (designated as CaDXR) was isolated and characterized for the first time from a medicinal Nyssaceae plant species, Camptotheca acuminata. The full-Length cDNA of CaDXR was 1823 bp containing a 1416 bp open reading frame (ORF) encoding a polypeptide of 472 amino acids. Comparative and bioinformatic analyses revealed that CaDXR showed extensive homology with DXRs from other plant species and contained a conserved

引用

页码：203 / 213

页数：11