Deubiquitinase USP37 Is Activated by CDK2 to Antagonize APCCDH1 and Promote S Phase Entry

被引:138
作者
Huang, XiaoDong [1 ]
Summers, Matthew K. [5 ]
Pham, Victoria [2 ]
Lill, Jennie R. [2 ]
Liu, Jinfeng [3 ]
Lee, Gwanghee [1 ]
Kirkpatrick, Donald S. [2 ]
Jackson, Peter K. [4 ]
Fang, Guowei [4 ]
Dixit, Vishva M. [1 ]
机构
[1] Genencor Inc, Dept Physiol Chem, San Francisco, CA 94080 USA
[2] Genencor Inc, Dept Prot Chem, San Francisco, CA 94080 USA
[3] Genencor Inc, Dept Bioinformat, San Francisco, CA 94080 USA
[4] Genencor Inc, Dept Cell Regulat, San Francisco, CA 94080 USA
[5] Lerner Res Inst, Dept Canc Biol, Cleveland, OH 44195 USA
关键词
CELL-CYCLE PROGRESSION; TAG SDS-PAGE; DEPENDENT PROTEOLYSIS; GENOMIC INSTABILITY; TUMOR SUPPRESSION; COMPLEX; INHIBITOR; MITOSIS; CDH1; EMI1;
D O I
10.1016/j.molcel.2011.03.027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell cycle progression requires the E3 ubiquitin ligase anaphase-promoting complex (APC/C), which uses the substrate adaptors CDC20 and CDH1 to target proteins for proteasomal degradation. The APC(CDH1) substrate cyclin A is critical for the G1/S transition and, paradoxically, accumulates even when APC(CDH1) is active. We show that the deubiquitinase USP37 binds CDH1 and removes degradative polyubiquitin from cyclin A. USP37 was induced by E2F transcription factors in G1, peaked at G1 IS, and was degraded in late mitosis. Phosphorylation of USP37 by CDK2 stimulated its full activity. USP37 overexpression caused premature cyclin A accumulation in G1 and accelerated S phase entry, whereas USP37 knock-down delayed these events. USP37 was inactive in mitosis because it was no longer phosphorylated by CDK2. Indeed, it switched from an antagonist to a substrate of APC(CDH1) and was modified with degradative K11-linked polyubiquitin.
引用
收藏
页码:511 / 523
页数:13
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