Effect of triacontanol on the micropropagation of Costus speciosus (Koen.) Sm. using rhizome thin sections

被引：10

作者：

Malabadi, RB ^{[1
]}

Mulgund, GS ^{[1
]}

Nataraja, K ^{[1
]}

机构：

[1] Karnatak Univ, Dept Bot, Div Plant Biotechnol, Dharwad 580003, Karnataka, India

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2005年 / 41卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

conservation; diosgenin; endangered plant; micropropagation; rhizome culture; spiral ginger;

D O I：

10.1079/IVP2004586

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The highest percentage of shoot regeneration of Costus speciosus was achieved using thin rhizome sections and triacontanol (TRIA). Factors affecting the rate of shoot multiplication and rooting with TRIA have been investigated. Initiation of shoot buds was observed when rhizome thin sections were cultured on B-5 basal medium supplemented with 5 mu g l(-1) TRIA. Shoots with two to three leaves produced roots when cultured on B-5 basal medium supplemented with 2 mu g l(-1) TRIA. The well-rooted shoots were hardened and transferred to soil where they showed normal growth and a 100% survival rate was achieved. Results of this study showed that TRIA can be used as an effective growth regulator in the micropropagation and conservation of C. speciosus.

引用

页码：129 / 132

页数：4

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