Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulosa cells?

被引：28
作者：
Karita, Masako [1 ]
Yamashita, Yoshiki [1 ]
Hayashi, Atsushi [1 ]
Yoshida, Yoko [1 ]
Hayashi, Mika [1 ]
Yamamoto, Hikaru [1 ]
Tanabe, Akiko [1 ]
Terai, Yoshito [1 ]
Ohmichi, Masahide [1 ]
机构：
[1] Osaka Med Coll, Dept Obstet & Gynecol, Osaka 5698686, Japan
来源：
FERTILITY AND STERILITY | 2011年 / 95卷 / 03期
关键词：
Endometriosis; IVF-ET; granulosa cell; PR; ER; real-time PCR; IN-VITRO FERTILIZATION; MESSENGER-RIBONUCLEIC-ACID; EMBRYO-TRANSFER; B-ISOFORM; A-ISOFORM; MECHANISMS; PROMOTER; ALPHA; TRANSCRIPTION; SUPERFAMILY;
D O I：
10.1016/j.fertnstert.2010.12.026
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: To evaluate how endometriosis affects the expression of estrogen and progesterone receptors mRNA in granulosa cells. Design: Prospective study. Setting: IVF-ET program at Osaka Medical College. Patient(s): Eighteen patients with revised American Fertility Society stage IV endometriosis and 23 patients with tubal infertility without endometriosis. Intervention(s): Granulosa cells obtained from patients with endometriosis were examined for estrogen (ER-beta, ER-alpha) and progesterone (PR-A, PR-B) receptor mRNA expression ratio against GAPDH. Main Outcome Measure(s): Hormonal environment and clinical outcome were investigated. Expression of ER and PR mRNA were evaluated by StepOne real-time polymerase chain reaction (PCR) analysis. Result(s): Total hMG/FSH levels were statistically higher in patients with endometriosis; however, high-quality embryo rates and pregnancy rates were lower in patients with endometriosis than in patients with tubal infertility. Expression of PR-A and ER-alpha in patients with endometriosis was statistically higher than in patients with tubal infertility. The expression of PRs and ERs in patients with tubal infertility showed a positive correlation; however, it was not identified in the endometriosis group. Conclusion(s): The higher PR-A and ER-alpha gene expression in granulosa cells in patients with endometriosis, compared with patients with tubal infertility, might be a leading cause of ovarian dysfunction due to endometriosis. (Fertil Steril (R) 2011; 95: 889-94. (C) 2011 by American Society for Reproductive Medicine.)
引用
收藏
页码：889 / 894
页数：6
相关论文
共 32 条
  • [1] The effect of endometriosis on implantation: Results from the Yale University in vitro fertilization and embryo transfer program
    Arici, A
    Oral, E
    Bukulmez, O
    Duleba, A
    Olive, DL
    Jones, EE
    [J]. FERTILITY AND STERILITY, 1996, 65 (03) : 603 - 607
  • [2] Outcome of IVF in patients with endometriosis in comparison with tubal-factor infertility
    Bergendal, A
    Naffah, S
    Nagy, C
    Bergqvist, A
    Sjoblom, P
    Hillensjo, T
    [J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1998, 15 (09) : 530 - 534
  • [3] Mechanisms of Disease Endometriosis
    Bulun, Serdar E.
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2009, 360 (03) : 268 - 279
  • [4] Gene expression analysis of endometrium reveals progesterone resistance and candidate susceptibility genes in women with endometriosis
    Burney, Richard O.
    Talbi, Said
    Hamilton, Amy E.
    Vo, Kim Chi
    Nyegaard, Mette
    Nezhat, Camran R.
    Lessey, Bruce A.
    Giudice, Linda C.
    [J]. ENDOCRINOLOGY, 2007, 148 (08) : 3814 - 3826
  • [5] PLASMA CONCENTRATION OF LH, FSH, PROLACTIN, PROGESTERONE AND ESTRADIOL-17BETA THROUGHOUT 4-DAY ESTROUS-CYCLE OF RAT
    BUTCHER, RL
    COLLINS, WE
    FUGO, NW
    [J]. ENDOCRINOLOGY, 1974, 94 (06) : 1704 - 1708
  • [6] Progesterone-dependent regulation of female reproductive activity by two distinct progesterone receptor isoforms
    Conneely, OM
    Mulac-Jericevic, B
    Lydon, JP
    [J]. STEROIDS, 2003, 68 (10-13) : 771 - 778
  • [7] Prevention of the polycystic ovarian phenotype and characterization of ovulatory capacity in the estrogen receptor-α knockout mouse
    Couse, JF
    Bunch, DO
    Lindzey, J
    Schomberg, DW
    Korach, KS
    [J]. ENDOCRINOLOGY, 1999, 140 (12) : 5855 - 5865
  • [8] Estrogen receptor-β is critical to granulosa cell differentiation and the ovulatory response to gonadotropins
    Couse, JF
    Yates, MM
    Deroo, BJ
    Korach, KS
    [J]. ENDOCRINOLOGY, 2005, 146 (08) : 3247 - 3262
  • [9] Progesterone Receptor B Recruits a Repressor Complex to a Half-PRE Site of the Estrogen Receptor α Gene Promoter
    De Amicis, F.
    Zupo, S.
    Panno, M. L.
    Malivindi, R.
    Giordano, F.
    Barone, I.
    Mauro, L.
    Fuqua, S. A. W.
    Ando, S.
    [J]. MOLECULAR ENDOCRINOLOGY, 2009, 23 (04) : 454 - 465
  • [10] Giangrande PH, 1999, RECENT PROG HORM RES, V54, P291
1234