Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulosa cells?

被引：28

作者：

Karita, Masako ^{[1
]}

Yamashita, Yoshiki ^{[1
]}

Hayashi, Atsushi ^{[1
]}

Yoshida, Yoko ^{[1
]}

Hayashi, Mika ^{[1
]}

Yamamoto, Hikaru ^{[1
]}

Tanabe, Akiko ^{[1
]}

Terai, Yoshito ^{[1
]}

Ohmichi, Masahide ^{[1
]}

机构：

[1] Osaka Med Coll, Dept Obstet & Gynecol, Osaka 5698686, Japan

来源：

FERTILITY AND STERILITY | 2011年 / 95卷 / 03期

关键词：

Endometriosis; IVF-ET; granulosa cell; PR; ER; real-time PCR; IN-VITRO FERTILIZATION; MESSENGER-RIBONUCLEIC-ACID; EMBRYO-TRANSFER; B-ISOFORM; A-ISOFORM; MECHANISMS; PROMOTER; ALPHA; TRANSCRIPTION; SUPERFAMILY;

D O I：

10.1016/j.fertnstert.2010.12.026

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To evaluate how endometriosis affects the expression of estrogen and progesterone receptors mRNA in granulosa cells. Design: Prospective study. Setting: IVF-ET program at Osaka Medical College. Patient(s): Eighteen patients with revised American Fertility Society stage IV endometriosis and 23 patients with tubal infertility without endometriosis. Intervention(s): Granulosa cells obtained from patients with endometriosis were examined for estrogen (ER-beta, ER-alpha) and progesterone (PR-A, PR-B) receptor mRNA expression ratio against GAPDH. Main Outcome Measure(s): Hormonal environment and clinical outcome were investigated. Expression of ER and PR mRNA were evaluated by StepOne real-time polymerase chain reaction (PCR) analysis. Result(s): Total hMG/FSH levels were statistically higher in patients with endometriosis; however, high-quality embryo rates and pregnancy rates were lower in patients with endometriosis than in patients with tubal infertility. Expression of PR-A and ER-alpha in patients with endometriosis was statistically higher than in patients with tubal infertility. The expression of PRs and ERs in patients with tubal infertility showed a positive correlation; however, it was not identified in the endometriosis group. Conclusion(s): The higher PR-A and ER-alpha gene expression in granulosa cells in patients with endometriosis, compared with patients with tubal infertility, might be a leading cause of ovarian dysfunction due to endometriosis. (Fertil Steril (R) 2011; 95: 889-94. (C) 2011 by American Society for Reproductive Medicine.)

引用

页码：889 / 894

页数：6

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