An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method

被引：10

作者：

Varughese, Eunice A. ^{[1
]}

Wymer, Larry J. ^{[1
]}

Haugland, Richard A. ^{[1
]}

机构：

[1] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 71卷 / 01期

关键词：

Bacillus; disinfection; MPN; robotics; recovery of injured spores;

D O I：

10.1016/j.mimet.2007.07.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using robotics and the MPN technique, a 96-microwell method was developed to compare two procedures for enumeration of viable chlorine-treated B. atrophaeus spores: broth-culture enrichment followed by real-time polymerase chain reaction analysis; and filter plating on agar. Recoveries of chlorine-treated spores were improved by broth enrichment over filter plating, whereas recoveries of non-treated spores were not different in the two procedures. Published by Elsevier B.V.

引用

页码：66 / 70

页数：5

共 27 条

[1]

ABSHIRE RL, 1980, APPL ENVIRON MICROB, V39, P395

[2] Responding to the threat of bioterrorism: A microbial ecology perspective - The case of anthrax [J].

Atlas R.M. .

International Microbiology, 2002, 5 (4) :161-167

[3] Forensic application of microbiological culture analysis to identify mail intentionally contaminated with Bacillus anthracis spores [J].

Beecher, Douglas J. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (08) :5304-5310

[4] Determination of the efficacy of two building decontamination strategies by surface sampling with culture and quantitative PCR analysis [J].

Buttner, MP ;

Cruz, P ;

Stetzenbach, LD ;

Klima-Comba, AK ;

Stevens, VL ;

Cronin, TD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (08) :4740-4747

[5] Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR [J].

Buttner, MP ;

Cruz-Perez, P ;

Stetzenbach, LD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2564-2570

[6] Phylogenetic analysis of Pacific salmon (Genus Oncorhynchus) based on mitochondrial DNA sequence data [J].

Domanico, MJ ;

Phillips, RB .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 1995, 4 (04) :366-371

[7] Molecular and phenotypic characterization of Bacillus thuringiensis isolated from leaves and insects [J].

Hansen, BM ;

Damgaard, PH ;

Eilenberg, J ;

Pedersen, JC .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1998, 71 (02) :106-114

[8]

Henchal EA, 2001, CLIN LAB MED, V21, P661

[9] A field investigation of Bacillus anthracis contamination of US Department of Agriculture and other Washington, DC, buildings during the anthrax attack of October 2001 [J].

Higgins, JA ;

Cooper, M ;

Schroeder-Tucker, L ;

Black, S ;

Miller, D ;

Karns, JS ;

Manthey, E ;

Breeze, R ;

Perdue, ML .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (01) :593-599

[10] DECISION AND DETECTION LIMITS FOR LINEAR CALIBRATION CURVES [J].

HUBAUX, A ;

VOS, G .

ANALYTICAL CHEMISTRY, 1970, 42 (08) :849-&

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