Immunoadsorption and subsequent immunoglobulin substitution decreases myocardial gene expression of desmin in dilated cardiomyopathy

被引:18
作者
Kallwellis-Opara, Angela [2 ]
Staudt, Alexander [2 ]
Trimpert, Christiane [2 ]
Noutsias, Michel [3 ]
Kuehl, Uwe [3 ]
Pauschinger, Matthias [3 ]
Schultheiss, Heinz-Peter [3 ]
Grube, Markus [4 ]
Boehm, Michael [5 ]
Baumann, Gert [6 ]
Voelker, Uwe [7 ]
Kroemer, Heyo K. [4 ]
Felix, Stephan B. [1 ]
机构
[1] Ernst Moritz Arndt Univ Greifswald, Klin Innere Med B, D-17475 Greifswald, Germany
[2] Ernst Moritz Arndt Univ Greifswald, Dept Internal Med B, Greifswald, Germany
[3] Charite Univ Med Berlin, Dept Cardiol & Pneumol, Berlin, Germany
[4] Ernst Moritz Arndt Univ Greifswald, Dept Pharmacol, Greifswald, Germany
[5] Univ Saarland, Dept Internal Med 3, D-6650 Homburg, Germany
[6] Charite Univ Med Berlin, Dept Cardiol Angiol & Pneumol, Berlin, Germany
[7] Ernst Moritz Arndt Univ Greifswald, Interfacultary Inst Genet & Funct Genom, Greifswald, Germany
来源
JOURNAL OF MOLECULAR MEDICINE-JMM | 2007年 / 85卷 / 12期
关键词
dilated cardiomyopathy; immunoadsorption; myocardial gene expression; desmin;
D O I
10.1007/s00109-007-0263-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Cardiac autoantibodies play a pathogenic role in dilated cardiomyopathy (DCM). Removal of antibodies by immunoadsorption (IA) induces hemodynamic improvement in DCM patients. The present study investigated the effects of IA on myocardial gene expression of the intermediate cytoskeletal filament desmin, which is upregulated in heart failure. RNA was isolated from five explanted non-failing hearts and five explanted failing hearts of DCM patients, and myocardial gene expression of desmin was estimated by real-time polymerase chain reaction (PCR). In a case-control study in six DCM patients (LVEF < 40%, NYHA II-III), IA and subsequent IgG substitution were performed at monthly intervals until month 3. Endomyocardial biopsies (EMBs) were obtained before and after IA (after 3-6 months). From six DCM patients without IA therapy (controls), EMBs were also obtained over a comparable time interval. Expression of the desmin gene was analyzed in these EMBs by real-time PCR. In failing explanted hearts, expression of desmin was significantly increased (0.88 +/- 0.12 vs 0.45 +/- 0.15 in non-failing hearts, P < 0.05). After IA, myocardial gene expression of desmin was significantly decreased (from 0.26 +/- 0.05 [baseline] to 0.14 +/- 0.04 [P < 0.05] vs baseline and controls). Removal of antibodies by IA modulates myocardial gene expression of desmin in DCM patients.
引用
收藏
页码:1429 / 1435
页数:7
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