Transcriptome Analysis of HepG2 Cells Expressing ORF3 from Swine Hepatitis E Virus to Determine the Effects of ORF3 on Host Cells

被引：9

作者：

Xu, Kailian ^{[1
]}

Guo, Shiyu ^{[1
]}

Zhao, Tianjing ^{[1
]}

Zhu, Huapei ^{[1
]}

Jiao, Hanwei ^{[1
]}

Shi, Qiaoyun ^{[1
]}

Pang, Feng ^{[1
]}

Li, Yaying ^{[1
]}

Li, Guohua ^{[1
]}

Peng, Dongmei ^{[1
]}

Nie, Xin ^{[1
]}

Cheng, Ying ^{[1
]}

Wu, Kebang ^{[1
]}

Du, Li ^{[1
]}

Cui, Ke ^{[2
]}

Zhang, Wenguang ^{[3
]}

Wang, Fengyang ^{[1
]}

机构：

[1] Hainan Univ, Coll Agr, Hainan Key Lab Trop Anim Reprod & Breeding & Epid, Anim Genet Engn Key Lab Haikou, Haikou 570228, Peoples R China

[2] Modern Agr Risk Warning & Prevent & Control Ctr H, Haikou 571100, Peoples R China

[3] Inner Mongolia Agr Univ, Coll Anim Sci, Inner Mongolia Key Lab Anim Genet Breeding & Repr, Inner Mongolia 010018, Hohhot, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2016年 / 2016卷

基金：

中国国家自然科学基金;

关键词：

LIPID-ACCUMULATION; PROTEIN; BIOLOGY; DKK1;

D O I：

10.1155/2016/1648030

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hepatitis E virus- (HEV-) mediated hepatitis has become a global public health problem. An important regulatory protein of HEV, ORF3, influences multiple signal pathways in host cells. In this study, to investigate the function of ORF3 from the swine form of HEV (SHEV), high-throughput RNA-Seq-based screening was performed to identify the differentially expressed genes in ORF3-expressing HepG2 cells. The results were validated with quantitative real-time PCR and gene ontology was employed to assign differentially expressed genes to functional categories. The results indicated that, in the established ORF3-expressing HepG2 cells, the mRNA levels of CLDN6, YLPM1, APOC3, NLRP1, SCARA3, FGA, FGG, FGB, and FREM1 were upregulated, whereas the mRNA levels of SLC2A3, DKK1, BPIFB2, and PTGR1 were downregulated. The deregulated expression of CLDN6 and FREM1 might contribute to changes in integral membrane protein and basement membrane protein expression, expression changes for NLRP1 might affect the apoptosis of HepG2 cells, and the altered expression of APOC3, SCARA3, and DKK1 may affect lipid metabolism in HepG2 cells. In conclusion, ORF3 plays a functional role in virus-cell interactions by affecting the expression of integral membrane protein and basement membrane proteins and by altering the process of apoptosis and lipid metabolism in host cells. These findings provide important insight into the pathogenic mechanism of HEV.

引用

页数：8

共 24 条

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