Ophiopogonin D ameliorates DNCB-induced atopic dermatitis-like lesions in BALB/c mice and TNF-α- inflamed HaCaT cell

被引:30
作者
An, Eun-Jin [1 ,2 ]
Kim, Yumi [1 ,2 ]
Lee, Seung-Hyeon [1 ,2 ]
Choi, Seung-Han [1 ,3 ]
Chung, Won Seok [1 ]
Jang, Hyeung-Jin [1 ,2 ,3 ]
机构
[1] Kyung Hee Univ, Coll Korean Med, 26 Kyungheedae Ro, Seoul 02447, South Korea
[2] Kyung Hee Univ, Grad Sch, Dept Sci Korean Med, Seoul, South Korea
[3] Kyung Hee Univ, Coll Korean Med, Grad Sch, Dept Biol Sci Korean Med, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
Ophiopogonin D; Atopic dermatitis; Inflammatory skin disease; Epidermal thickness; Allergic contact dermatitis; HaCaT cells; SKIN-LESIONS; EXPRESSION; INFLAMMATION; EOTAXIN; TARGETS; RADIX;
D O I
10.1016/j.bbrc.2019.10.190
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Atopic dermatitis (AD) can occur in both children and adults, and the symptoms include itching and eczema, which in turn cause patients to suffer. Ophiopogonin D (OP-D) is a steroidal glycoside from Radix Ophiopogon japonicus, which is well known as an effective anti-inflammatory herbal medicine in many Asian countries. In this study, we aimed to investigate the anti-inflammatory effects of OP-D, using an AD mouse model and inflamed HaCaT cells. Through a histopathological analysis, we were able to confirm the suppressive effects of OP-D on skin thickening and the mast cell activation in AD-like mouse back skin tissues stimulated by DNCB. In addition, we detected significant decreases in cytokine expression levels through multiplex assessment assays of the OP-D-treated mice blood. We observed the anti-inflammatory effect of OP-D in the spleen, causing weight loss in the spleen and in the mRNA expression levels related to diverse cytokines. In human keratinocytes inflamed by TNF-alpha, OP-D inhibited p38 and ERK protein activation and showed a reduction of NF-kappa B nuclear translocation. Furthermore, OP-D attenuated pro-inflammatory cytokine mRNA expressions in TNF-alpha-inflamed HaCaT cells. Accordingly, we came to the conclusion that OP-D is a potential natural drug which can be used in order to treat inflammatory skin diseases, such as AD. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码:40 / 46
页数:7
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