Development of an inducible gene expression system for Lactobacillus sakei

被引：37
作者：
Axelsson, L [1 ]
Lindstad, G [1 ]
Naterstad, K [1 ]
机构：
[1] Norwegian Food Res Inst, MATFORSK, N-1430 As, Norway
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2003年 / 37卷 / 02期
关键词：
Lactobacillus; controlled expression; vector; bacteriocin;
D O I：
10.1046/j.1472-765X.2003.01360.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production. Methods and Results: A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters. Conclusion: The pKRV3 system can be used as an inducible gene expression system in lactobacilli. Significance and Impact of the Study: A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.
引用
收藏
页码：115 / 120
页数：6
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