Graphene-DNAzyme junctions: a platform for direct metal ion detection with ultrahigh sensitivity

被引：41

作者：

Gao, Li ^{[1
]}

Li, Le-Le ^{[2
]}

Wang, Xiaolong ^{[1
]}

Wu, Peiwen ^{[2
]}

Cao, Yang ^{[1
]}

Liang, Bo ^{[3
]}

Li, Xin ^{[3
]}

Lin, Yuanwei ^{[1
]}

Lu, Yi ^{[2
]}

Guo, Xuefeng ^{[1
,4
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Nanochem,Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[3] Adesso Adv Mat Wuxi Co Ltd, Wuxi 214000, Jiangsu, Peoples R China

[4] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China

来源：

CHEMICAL SCIENCE | 2015年 / 6卷 / 04期

基金：

美国国家卫生研究院;

关键词：

SINGLE-MOLECULE DETECTION; CATALYTIC BEACON SENSOR; IN-VITRO SELECTION; FLUORESCENT SENSOR; AQUEOUS-SOLUTION; LABEL-FREE; DNA; BIOSENSOR; PROTEINS; CU2+;

D O I：

10.1039/c4sc03612c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Many metal ions are present in biology and in the human body in trace amounts. Despite numerous efforts, metal sensors with ultrahigh sensitivity (<a few picomolar) are rarely achieved. Here, we describe a platform method that integrates a Cu2+-dependent DNAzyme into graphene-molecule junctions and its application for direct detection of paramagnetic Cu2+ with femtomolar sensitivity and high selectivity. Since DNAzymes specific for other metal ions can be obtained through in vitro selection, the method demonstrated here can be applied to the detection of a broad range of other metal ions.

引用

页码：2469 / 2473

页数：5

共 53 条

[1] DNA enzymes [J].