Transmission of Raccoon-Passaged Chronic Wasting Disease Agent to White-Tailed Deer

被引:5
作者
Cassmann, Eric D. [1 ]
Frese, Alexis J. [1 ,2 ]
Moore, S. Jo [1 ,2 ]
Greenlee, Justin J. [1 ]
机构
[1] ARS, USDA, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA
[2] Oak Ridge Inst Sci & Educ, 1299 Bethel Valley Rd, Oak Ridge, TN 37830 USA
来源
VIRUSES-BASEL | 2022年 / 14卷 / 07期
关键词
chronic wasting disease; interspecies transmission; prion; transmissible spongiform encephalopathy; white-tailed deer; PRIONS;
D O I
10.3390/v14071578
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transmission characteristics of prion diseases are influenced by host prion protein sequence and, therefore, the host species. Chronic wasting disease (CWD), a prion disease of cervids, has widespread geographical distribution throughout North America and occurs in both wild and farmed populations. CWD prions contaminate the environment through scattered excrement and decomposing carcasses. Fresh carcasses with CWD prions are accessible by free-ranging mesopredators such as raccoons and may provide a route of exposure. Previous studies demonstrated the susceptibility of raccoons to CWD from white-tailed deer. In this study, we demonstrate that white-tailed deer replicate raccoon-passaged CWD prions which results in clinical disease similar to intraspecies CWD transmission. Six white-tailed deer were oronasally inoculated with brain homogenate from a raccoon with CWD. All six deer developed clinical disease, had widespread lymphoid distribution of misfolded CWD prions (PrPSc), and had neuropathologic lesions with PrPSc accumulation in the brain. The presence of PrPSc was confirmed by immunohistochemistry, enzyme-linked immunoassay, and western blot. The western blot migration pattern of raccoon-passaged CWD was different from white-tailed deer CWD. Transmission of raccoon CWD back to white-tailed deer resulted in an interposed molecular phenotype that was measurably different from white-tailed deer CWD.
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页数:12
相关论文
共 26 条
[1]   Interspecies transmission of prions [J].
Afanasieva, E. G. ;
Kushnirov, V. V. ;
Ter-Avanesyan, M. D. .
BIOCHEMISTRY-MOSCOW, 2011, 76 (13) :1375-1384
[2]   Second passage of chronic wasting disease of mule deer to sheep by intracranial inoculation compared to classical scrapie [J].
Cassmann, Eric D. ;
Frese, Rylie D. ;
Greenlee, Justin J. .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2021, 33 (04) :711-720
[3]   Insights into Chronic Wasting Disease and Bovine Spongiform Encephalopathy Species Barriers by Use of Real-Time Conversion [J].
Davenport, Kristen A. ;
Henderson, Davin M. ;
Bian, Jifeng ;
Telling, Glenn C. ;
Mathiason, Candace K. ;
Hoover, Edward A. .
JOURNAL OF VIROLOGY, 2015, 89 (18) :9524-9531
[4]  
Ferrer I, 2018, HAND CLINIC, V145, P79, DOI 10.1016/B978-0-12-802395-2.00007-9
[5]   Detection of Chronic Wasting Disease Prions in Salivary, Urinary, and Intestinal Tissues of Deer: Potential Mechanisms of Prion Shedding and Transmission [J].
Haley, Nicholas J. ;
Mathiason, Candace K. ;
Carver, Scott ;
Zabel, Mark ;
Telling, Glenn C. ;
Hoover, Edward A. .
JOURNAL OF VIROLOGY, 2011, 85 (13) :6309-6318
[6]   Experimental transmission of US scrapie agent by nasal, peritoneal, and conjunctival routes to genetically susceptible sheep [J].
Hamir, A. N. ;
Kunkle, R. A. ;
Richt, J. A. ;
Miller, J. M. ;
Greenlee, J. J. .
VETERINARY PATHOLOGY, 2008, 45 (01) :7-11
[7]  
Howerth EW, 2018, PATHOLOGY OF WILDLIFE AND ZOO ANIMALS, P149, DOI 10.1016/B978-0-12-805306-5.00006-7
[8]   The emergence of classical BSE from atypical/Nor98 scrapie [J].
Huor, Alvina ;
Carlos Espinosa, Juan ;
Vidal, Enric ;
Cassard, Herve ;
Douet, Jean-Yves ;
Lugan, Severine ;
Aron, Naima ;
Marin-Moreno, Alba ;
Lorenzo, Patricia ;
Aguilar-Calvo, Patricia ;
Badiola, Juan ;
Bolea, Rosa ;
Pumarola, Marti ;
Benestad, Sylvie L. ;
Orge, Leonore ;
Thackray, Alana M. ;
Bujdoso, Raymond ;
Torres, Juan-Maria ;
Andreoletti, Olivier .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (52) :26853-26862
[9]   Prion Protein Polymorphisms Affect Chronic Wasting Disease Progression [J].
Johnson, Chad J. ;
Herbst, Allen ;
Duque-Velasquez, Camilo ;
Vanderloo, Joshua P. ;
Bochsler, Phil ;
Chappell, Rick ;
McKenzie, Debbie .
PLOS ONE, 2011, 6 (03)
[10]   Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie [J].
Marin, Belen ;
Otero, Alicia ;
Lugan, Severine ;
Carlos Espinosa, Juan ;
Marin-Moreno, Alba ;
Vidal, Enric ;
Hedman, Carlos ;
Romero, Antonio ;
Pumarola, Marti ;
Badiola, Juan J. ;
Maria Torres, Juan ;
Andreoletti, Olivier ;
Bolea, Rosa .
SCIENTIFIC REPORTS, 2021, 11 (01)