Structural modification of a periodic polypeptide through biosynthetic replacement of proline with azetidine-2-carboxylic acid

被引：40
作者：
Deming, TJ
Fournier, MJ
Mason, TL
Tirrell, DA
机构：
[1] UNIV MASSACHUSETTS, DEPT POLYMER SCI & ENGN, AMHERST, MA 01003 USA
[2] UNIV MASSACHUSETTS, DEPT BIOCHEM & MOLEC BIOL, AMHERST, MA 01003 USA
来源：
MACROMOLECULES | 1996年 / 29卷 / 05期
关键词：
D O I：
10.1021/ma9510698
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Repetitive polypeptides comprising 16 repeats of the sequence -(AlaGly)(3)ProGluGly- (1a) have been prepared from Escherichia coli as overexpressed recombinant proteins. Partial in vivo replacement of the proline (Pro) residues in sequence 1a with L-azetidine-2-carboxylic acid (Ate) was achieved by expression of the target protein in medium containing Ate and lacking Pro. NMR and amino acid analysis for residual proline in the polymer indicated 25-40% replacement of Pro by Ate. While polymers of la form conformationally disordered solids, incorporation of Aze allows this material to adopt a beta-sheet structure in the solid state.
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页码：1442 / 1444
页数：3
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