Optimization of a nonradioactive immunosorbent assay for p38α mitogen-activated protein kinase activity

被引：35

作者：

Goettert, Marcia ^{[1
]}

Graeser, Ralph ^{[2
]}

Laufer, Stefan A. ^{[1
]}

机构：

[1] Univ Tubingen, Dept Pharmaceut & Med Chem, Inst Pharm, D-72076 Tubingen, Germany

[2] ProQinase GmbH, D-79106 Freiburg, Germany

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 406卷 / 02期

关键词：

MONOCLONAL-ANTIBODIES; P38; INHIBITOR; MECHANISM; TARGETS; PATHWAY;

D O I：

10.1016/j.ab.2010.07.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe an optimization of a nonradioactive immunosorbent p38 alpha mitogen-activated protein kinase (MAPK) activity assay to determine inhibitory potency of small molecule inhibitors. The assay omits the secondary antibody and, therefore, is shorter, more accurate, and easier to handle (total assay time of 3 h). This direct assay uses a new monoclonal anti-phospho-ATF-2 (Thr69/71) peroxidase-conjugated antibody, increasing specificity and eliminating problems with cross-reactivities of secondary antibodies. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：233 / 234

页数：2

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