Determination of apatinib and its three active metabolites by UPLC-MS/MS in a Phase IV clinical trial in NSCLC patients

被引：3

作者：

Guan, Shaoxing ^{[1
]}

Shi, Wei ^{[2
,3
,4
]}

Zhao, Zerui ^{[2
]}

Wang, Fei ^{[5
]}

Jiang, Fulin ^{[1
]}

Zhang, Caibin ^{[1
]}

Jin, Zhongming ^{[1
]}

Guan, Yanping ^{[1
]}

Liu, Dihan ^{[2
]}

Zhong, GuoPing ^{[1
]}

Huang, Min ^{[1
]}

Long, Hao ^{[2
]}

Wang, Xueding ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Lab Drug Metab & Pharmacokinet, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Dept Thorac Oncol, 651 Dongfeng East Rd, Guangzhou 510080, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med,Dept Med Oncol, 651 Dongfeng East Rd, Guangzhou 510080, Guangdong, Peoples R China

[4] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China

[5] Qingxi Hosp, Dept Pharm, Dongguan 510080, Guangdong, Peoples R China

来源：

BIOANALYSIS | 2019年 / 11卷 / 22期

基金：

美国国家科学基金会;

关键词：

active metabolites; apatinib; therapeutic drug monitoring; UPLC-MS/MS; GASTRIC-CANCER; INHIBITOR;

D O I：

10.4155/bio-2019-0214

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: To develop and validate a simple method using UPLC-MS/MS for determination of apatinib and its three active metabolites in a Phase IV clinical trial. Materials & methods: All compounds were separated on a Hypersil GOLD (TM) aQ C18 Polar Endcapped LC column (50 x 2.1 mm, 1.9 mu m, Thermo) using 5 mmol/l ammonium acetate with 0.1% formic acid:acetonitrile (20:80, v/v) as the mobile phase after a rapid liquid-liquid extraction. This method was validated over the linear concentration range of 1.00-1000 ng/ml for each compound. Results: The interassay precision and accuracy were less than +/- 15%. The validated method was successfully applied to determine concentrations of clinical samples in non-small-cell lung cancer patients.

引用

页码：2049 / 2060

页数：12

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