Breast cancer drug delivery by novel drug-loaded chitosan-coated magnetic nanoparticles

被引:55
作者
Taherian, A. [1 ]
Esfandiari, N. [1 ]
Rouhani, S. [2 ]
机构
[1] Shahid Beheshti Univ, Fac Life Sci & Biotechnol, Tehran, Iran
[2] Inst Color Sci & Technol, Dept Organ Colorants, Tehran, Iran
关键词
Chitosan-coated magnetic nanoparticles; Black pomegranate peel extract; Drug delivery; Breast cancer; PUNICA-GRANATUM POMEGRANATE; PEEL EXTRACT; ANTIOXIDANT ACTIVITY; DOXORUBICIN; CONJUGATE; STABILITY; PHENOLICS; RELEASE;
D O I
10.1186/s12645-021-00086-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background Breast cancer is one of the most challenging cancers among women which is considered one of the most lethal cancers to this date. From the time that cancer has been discovered, finding the best therapeutic method is still an ongoing process. As a novel therapeutic method, nanomedicine has brought a vast number of materials that could versatilely be used as a drug carrier. The purpose of this study is to develop a novel black pomegranate peel extract loaded with chitosan-coated magnetic nanoparticles to treat breast cancer cells. Results The morphology and size distribution of the nanoparticles studied by dynamic light scattering, atomic force microscopy, scanning, and transitional electron microscopy showed the spherical shape of the nanoparticles and their promising size range. Studies by Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer, and zeta sizer confirmed the synthesis, substantial crystallinity, magnetic potential of the nanoparticles, and their satisfactory stability. The DPPH assay revealed that the obtained black pomegranate peel extract has 60% free radical scavenging activity. The cytotoxicity studies by MTT and LDH assay carried out on NIH/3T3, MBA-MB-231, and 4T1 cells confirmed that the magnetic nanoparticles had no significant cytotoxicity on the cells. However, the drug-loaded nanoparticles could significantly eradicate cancerous cells which had more efficiency comparing to free drug. Furthermore, free drug and drug-loaded nanoparticles had no toxic effect on normal cells. Conclusion Owing to the results achieved from this study, the novel drug-loaded nanoparticles are compatible to be used for breast cancer treatment and could potentially be used for further in vivo studies.
引用
收藏
页数:20
相关论文
共 83 条
[1]   Antioxidant and anticancer activities of chamomile (Matricaria recutita L.) [J].
Al-Dabbagh, Bayan ;
Elhaty, Ismail A. ;
Elhaw, Mohamed ;
Murali, Chandraprabha ;
Al Mansoori, Ameera ;
Awad, Basma ;
Amin, Amr .
BMC RESEARCH NOTES, 2019, 12 (1)
[2]   Phenolic Compounds of Pomegranate Byproducts (Outer Skin, Mesocarp, Divider Membrane) and Their Antioxidant Activities [J].
Ambigaipalan, Priyatharini ;
de Camargo, Adriano Costa ;
Shahidi, Fereidoon .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (34) :6584-6604
[3]   Chitosan magnetic nanoparticles for drug delivery systems [J].
Assa, Farnaz ;
Jafarizadeh-Malmiri, Hoda ;
Ajamein, Hossein ;
Vaghari, Hamideh ;
Anarjan, Navideh ;
Ahmadi, Omid ;
Berenjian, Aydin .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 2017, 37 (04) :492-509
[4]   Physicochemical and Phytochemical Characterization and Storage Stability of Freeze-dried Encapsulated Pomegranate Peel Anthocyanin and In Vitro Evaluation of Its Antioxidant Activity [J].
Azarpazhooh, Elham ;
Sharayei, Parvin ;
Zomorodi, Shahin ;
Ramaswamy, Hosahalli S. .
FOOD AND BIOPROCESS TECHNOLOGY, 2019, 12 (02) :199-210
[5]  
Benguiar R., 2020, INT J BIOSCI, V16, P35, DOI [10.12692/ijb/16.6.35-44, DOI 10.12692/ijb/16.6.35-44]
[6]   Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications [J].
Berger, J ;
Reist, M ;
Mayer, JM ;
Felt, O ;
Peppas, NA ;
Gurny, R .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2004, 57 (01) :19-34
[7]   Nanoparticle-Based Medicines: A Review of FDA-Approved Materials and Clinical Trials to Date [J].
Bobo, Daniel ;
Robinson, Kye J. ;
Islam, Jiaul ;
Thurecht, Kristofer J. ;
Corrie, Simon R. .
PHARMACEUTICAL RESEARCH, 2016, 33 (10) :2373-2387
[8]   Comparison of scanning electron microscopy, dynamic light scattering and analytical ultracentrifugation for the sizing of poly(butyl cyanoacrylate) nanoparticles [J].
Bootz, A ;
Vogel, V ;
Schubert, D ;
Kreuter, J .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2004, 57 (02) :369-375
[9]  
Bray F, 2018, CA-CANCER J CLIN, V68, P394, DOI [10.3322/caac.21492, 10.3322/caac.21609]
[10]   Chitosan nanoparticles conjugate with trypsin and trypsin inhibitor [J].
Chanphai, P. ;
Tajmir-Riahi, H. A. .
CARBOHYDRATE POLYMERS, 2016, 144 :346-352