Production and characterisation of thermostable alkaline protease from Bacillus subtilis isolated from LA hot spring, Terengganu

被引:0
|
作者
Maslinda, Alias [1 ]
Hakim, Che Harun Mohammad [1 ,4 ]
Ashraf, Razali Nurul [1 ]
Jasnizat, Saidin [2 ]
Nazaitulshila, Rasit [1 ]
Munirah, Mohd Zaideen Izyan [3 ]
Sofiah, Hamzah [1 ]
机构
[1] Univ Malaysia Terengganu, Fac Ocean Engn Technol & Informat, Kuala Terengganu 21300, Kuala Terenggan, Malaysia
[2] Univ Malaysia Terengganu, Fac Sci & Marine Environm, Kuala Terengganu 21300, Kuala Terenggan, Malaysia
[3] Univ Malaysia Terengganu, Fac Maritime Management, Kuala Terengganu 21300, Kuala Terenggan, Malaysia
[4] Univ Sheffield, Dept Chem & Biol Engn, Sheffield S1 3JD, S Yorkshire, England
来源
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2021年 / 16卷 / 07期
关键词
Thermostable protease; Bacillus subtilis; protease activity; alkaline protease; enzyme;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This research aims to produce thermostable alkaline protease from Bacillus subtilis isolated from La Hot Spring, Terengganu, Malaysia. The study was also conducted to determine the optimum conditions for protease production and stability by considering several parameters including pH, temperature and salt concentration. All seven bacteria were screened on skim milk agar overnight at 37 degrees C. Three strains with the highest proteolytic activity were identified in protease specific medium. The thermostable alkaline protease had an optimum temperature of 60 degrees C which achieved 85.73, 82.90 and 83.05 U/mL of protease activity for the three strains respectively. Furthermore, the strains exhibited significant activity of more than 90% from their original activity. Meanwhile, the optimum pH for protease production was pH 9 with the protease activity of 76.76, 79.71 and 88.39 U/mL for TB4, TB6 and TB9 strains, respectively. Proteases were found stable at pH 9 where the loss did not exceed 30% of its original activity. Collectively, all of the data emphasised that proteases from B. subtilis were alkaline thermostable proteases in accordance with a recent report. The finding highlights the viability of the proteases for biotechnological and industrial applications.
引用
收藏
页码:84 / 91
页数:8
相关论文
共 50 条
  • [41] A report on thermostable alkaline protease producing bacteria from a terrestrial thermal spring
    Pathak, Anupama P.
    Rathod, Mukundraj G.
    INDIAN JOURNAL OF GEO-MARINE SCIENCES, 2015, 44 (07) : 1104 - 1111
  • [42] STABILITY AND ACTIVITY PROFILE OF ALKALINE PROTEASE, PRODUCED FROM BACILLUS SUBTILIS
    Anjum, Sobia
    Mukhtar, Hamid
    Nawaz, Ali
    Iftikhar, Tehreema
    Ikram-Ul-Haq
    PAKISTAN JOURNAL OF BOTANY, 2015, 47 (02) : 771 - 777
  • [43] A study of extracellular alkaline protease from Bacillus subtilis NCIM 2713
    Mane, R.R.
    Bapat, M.M.
    Indian Journal of Experimental Biology, 2001, 39 (06) : 578 - 583
  • [44] Production and characterization of thermostable metallo-keratinase from newly isolated Bacillus subtilis NRC 3
    Tork, Sanaa E.
    Shahein, Yasser E.
    El-Hakim, Amr E.
    Abdel-Aty, Azza M.
    Aly, Magda M.
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 55 : 169 - 175
  • [45] Production of alkaline protease by a halotolerant bacillus pumilus isolated from Van Lake in Turkey
    Ates, O
    Kazan, D
    Oner, ET
    JOURNAL OF BIOTECHNOLOGY, 2005, 118 : S94 - S95
  • [46] Thermostable Alkaline Protease Production by Bacillus Aryabhattai J4
    Selim, Samy
    FASEB JOURNAL, 2015, 29
  • [47] Thermostable Alkaline Protease from a Novel Marine Haloalkalophilic Bacillus Clausii Isolate
    C. Ganesh Kumar
    Han-Seung Joo
    Yoon-Mo Koo
    Seung R. Paik
    Chung-Soon Chang
    World Journal of Microbiology and Biotechnology, 2004, 20 : 351 - 357
  • [48] Thermostable alkaline protease from a novel marine haloalkalophilic Bacillus clausii isolate
    Kumar, CG
    Joo, HS
    Koo, YM
    Paik, SR
    Chang, CS
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2004, 20 (04): : 351 - 357
  • [49] Production and immobilization of alkaline protease from Bacillus mycoides
    Abdel-Naby, MA
    Ismail, AMS
    Ahmed, SA
    Fattah, AFA
    BIORESOURCE TECHNOLOGY, 1998, 64 (03) : 205 - 210
  • [50] Cloning and expression of thermostable protease gene from Bacillus stearothermophilus HY-69 in Bacillus subtilis
    Yang, SJ
    Jin, C
    Bao, DH
    Zhang, SZ
    RECOMBINANT DNA BIOTECHNOLOGY III: THE INTEGRATION OF BIOLOGICAL AND ENGINEERING SCIENCES, 1996, 782 : 219 - 225