Multilevel Calcium Phosphate Coating: a Model of Bone-Like Topography for Osteoimmunology

被引：0

作者：

Litvinova, L. S. ^{[1
]}

Malashchenko, V. V. ^{[1
]}

Shunkin, E. O. ^{[1
]}

Shupletsova, V. V. ^{[1
]}

Khaziakhmatova, O. G. ^{[1
]}

Yurova, K. A. ^{[1
]}

Melashchenko, E. S. ^{[1
]}

Khlusov, I. A. ^{[1
,2
,3
]}

Komarova, E. G. ^{[4
]}

Chebodaeva, V. V. ^{[4
]}

Sharkeev, Yu P. ^{[2
,4
]}

机构：

[1] Immanuel Kant Baltic Fed Univ, Kaliningrad 236000, Russia

[2] Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia

[3] Siberian State Med Univ, Tomsk 634050, Russia

[4] Inst Strength Phys & Mat Sci SB RAS, Tomsk 634055, Russia

来源：

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON PHYSICAL MESOMECHANICS. MATERIALS WITH MULTILEVEL HIERARCHICAL STRUCTURE AND INTELLIGENT MANUFACTURING TECHNOLOGY | 2020年 / 2310卷

基金：

俄罗斯科学基金会;

关键词：

ADIPOSE-TISSUE; STROMAL CELLS; RELIEF;

D O I：

10.1063/5.0034409

中图分类号：

TH [机械、仪表工业];

学科分类号：

0802 ;

摘要：

Osteoimmunolgy describes the interactions between blood immune cells and human multipotent mesenchymal stromal cells (hMMSCs) as a basis of successful wound and fracture healing. The aim was to investigate in vitro interaction of adipose-derived hMMSCs and human blood mononuclear cells (hBMNCs) modulated by calcium phosphate (CaP) coating in 3D culture. Titanium plates (10 x 10 x 1 mm(3)) with micro-arc bilateral multilevel CaP coatings were used. Cell-IQ phase-contrast microscopy and the real-time cell analyzer (RTCA) showed the CaP coating and hBMNCs synergistic negative effects on hMIVISC motility that could he conditioned by enhanced osteogenic differentiation of stromal cells. Indeed, a 10-fold increase in the bone mineralization around the CaP-coated samples was detected in the mixed (hBMNCs + hMMSCs) 3D culture. Thus, cellular and molecular crosstalk between hBMNCs and hMMSCs modulated by multilevel micro-arc CaP coating with hone-like topography is an effective 3D model to study in vitro the novel pathways of osteoimmunology.

引用

页数：5

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